miRNA profiling of human naive CD4 T cells links miR-34c-5p to cell activation and HIV replication.


Cell activation is a vital step for T-cell memory/effector differentiation as well as for productive HIV infection. To identify novel regulators of this process, we used next-generation sequencing to profile changes in microRNA expression occurring in purified human naive CD4 T cells in response to TCR stimulation and/or HIV infection. Our results demonstrate, for the first time, the transcriptional up-regulation of miR-34c-5p in response to TCR stimulation in naive CD4 T cells. The induction of this miR was further consistently found to be reduced by both HIV-1 and HIV-2 infections. Overexpression of miR-34c-5p led to changes in the expression of several genes involved in TCR signaling and cell activation, confirming its role as a novel regulator of naive CD4 T-cell activation. We additionally show that miR-34c-5p promotes HIV-1 replication, suggesting that its down-regulation during HIV infection may be part of an anti-viral host response.

SEEK ID: https://fairdomhub.org/publications/429

PubMed ID: 27993935

Projects: miRiAD - exploring the role of microRNAs in T cell function and anti-vir...

Publication type: Not specified

Journal: EMBO J

Citation: EMBO J. 2017 Feb 1;36(3):346-360. doi: 10.15252/embj.201694335. Epub 2016 Dec 19.

Date Published: 1st Feb 2017

Registered Mode: Not specified

Authors: A. J. Amaral, J. Andrade, R. B. Foxall, P. Matoso, A. M. Matos, R. S. Soares, C. Rocha, C. G. Ramos, R. Tendeiro, A. Serra-Caetano, J. A. Guerra-Assuncao, M. Santa-Marta, J. Goncalves, M. Gama-Carvalho, A. E. Sousa


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Created: 5th Nov 2019 at 18:35

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