UPARSE: highly accurate OTU sequences from microbial amplicon reads

Abstract:

Amplified marker-gene sequences can be used to understand microbial community structure, but they suffer from a high level of sequencing and amplification artifacts. The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with =1% incorrect bases in artificial microbial community tests, compared with >3% incorrect bases commonly reported by other methods. The improved accuracy results in far fewer OTUs, consistently closer to the expected number of species in a community.

SEEK ID: https://fairdomhub.org/publications/265

PubMed ID: 23955772

Projects: GenoSysFat

Publication type: Not specified

Journal: Nat Methods

Citation: Nat Methods. 2013 Oct;10(10):996-8. doi: 10.1038/nmeth.2604. Epub 2013 Aug 18.

Date Published: 18th Aug 2013

Registered Mode: Not specified

Author: R. C. Edgar

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Created: 8th Jul 2016 at 09:45

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