SOPs

This is a well-established, classical genetic method of constructing chromosomal monolysogenic fusions to a promoterless lacZ gene.

This SOP describes the SUMO procedure for determining B-galactosidase activities.

This document describes the chemostat conditions to run comparable experiments in different bioreactors in different labs.

This file describes how to isolate mRNA from E. coli using the kit from Epicentre, for gene expression analysis via RT-PCR

This SOP describes the preparation of E. coli RNA

This protocol describes the transcriptional profiling of E. coli cultures using microarrays. The protocol utilises RNA isolated as described in another SOP (SUMO RNA isolation from E. coli) and with hybridisation to Ocimum Ocichip E. coli K-12 microarrays.