Assays

What is an Assay?
173 Assays visible to you, out of a total of 173

SDtoLD 260410

Property Value
BioDare ID 13982031569136
Author Laura Dixon
Institution University of Edinburgh
License CC_BY

Description

pTOC plate 0683 pCAB plate0684 LHY plate 0685 control plate 0686 TOC plate0687 pLHY plate 0688

Purpose

Re-entrainment dynamics during the transition between light:dark cycles with different photoperiods, comparing Arabidopsis thaliana and Ostreococcus tauri. This expt in O. tauri ...

Edwards et al. (2010); WT in different light conditions.

Property Value
BioDare ID 13227622661196
Author Edwards, K.D.
Institution University of Edinburgh
License CC_BY

Description

Literature data from: 'Quantitative analysis of regulatory flexibility under changing environmental conditions.' by: Edwards, K.D..

Theory suggested that multiple loops contribute the flexibility for molecular rhythms to track multiple phases ...

Effects of GA on clock in WT and prr9prr7

Property Value
BioDare ID 3838
Author Daniel Seaton
Institution University of Edinburgh
License CC_BY

Description

Purpose

To test whether GA has any effect on circadian clock period in the WT, and whether this effect is altered in the prr9prr7 mutant.

Comments

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Chew Yin Hoon

Study: Chew et al. (2022)

Flis et al. TiMet clock gene comparison across photoperiods

Property Value
BioDare ID 3967
Author Anna Flis
Institution Max Planck Inst. Golm
License CC_BY

Description

Literature data from: 'Photoperiod-dependent changes in the phase of core clock transcripts and global transcriptional outputs at dawn and dusk in Arabidopsis' by: Anna Flis.

Col plants were grown in soil under 6, 8, 12 and 18h photoperiod for 21 days, and ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Flis Anna

Study: Flis et al. (2016)

Q-PCR LD (12:12) 17C LHY

Property Value
BioDare ID 3492
Author Aurora Pinas-Fernandez
Institution University of Edinburgh
License CC_BY

Description

Literature data from: 'Temp' by: Aurora Pinas-Fernandez.

Wild-type and clock-mutant seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees, every 2h, for 24h.

Purpose

...

McWatters A.t. Col-0 WT clock RNA timeseries

Property Value
BioDare ID 3488
Author Harriet G. McWatters
Institution University of Oxford
License CC_BY

Description

Literature data from: 'Defining the robust behaviour of the plant clock gene circuit with absolute RNA timeseries and open infrastructure.' by: Harriet G. McWatters.

data are col0 8 d seedlings grown on sucrose in either LD 12:12 at 20 oC or WC 27:12 in LL. Gene ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Flis Anna

Study: Flis et al. (2015)

Clock gene RNAs under RD-RR in clock mutants, expt 4

Property Value
BioDare ID 13228357183121
Author Locke
Institution
License

Description

Literature data from: 'Extension of a genetic network model by iterative experimentation and mathematical analysis.' by: Locke.

First, heroic large-scale RNA profile screen by real-time qPCR in 96-well format, by Megan Southern in 2005. Experiment 4 of 4.

Purpose

Testing effects of clock ...

Clock gene RNAs under RD-RR in clock mutants, expt 3

Property Value
BioDare ID 13228357055348
Author Locke
Institution
License

Description

Literature data from: 'Extension of a genetic network model by iterative experimentation and mathematical analysis.' by: Locke.

First, heroic large-scale RNA profile screen by real-time qPCR in 96-well format, by Megan Southern in 2005. Experiment 3 of 4.

Purpose

Testing effects of clock ...

Clock gene RNAs under RD-RR in clock mutants, expt 2

Property Value
BioDare ID 13227619871305
Author Locke
Institution
License

Description

Literature data from: 'Extension of a genetic network model by iterative experimentation and mathematical analysis.' by: Locke.

First, heroic large-scale RNA profile screen by real-time qPCR in 96-well format, by Megan Southern in 2005. Experiment 2 of 4.

Purpose

Testing effects of clock ...

Clock gene RNAs under RD-RR in clock mutants, expt 1

Property Value
BioDare ID 13228298288040
Author Locke
Institution University of Warwick
License

Description

Literature data from: 'Extension of a genetic network model by iterative experimentation and mathematical analysis.' by: Locke.

First, heroic large-scale RNA profile screen by real-time qPCR in 96-well format, by Megan Southern in 2005. Data are noisy (especially ...

Q-PCR LD (12:12) 17C ELF3

Property Value
BioDare ID 12962294335805
Author Aurora Pinas-Fernandez
Institution University of Edinburgh
License

Description

Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.

Purpose

Experiment carry out to reparameterize the clock off Succrose

Comments

Sample time 24h. 24h sample ...

Q-PCR LD (12:12) 17C ELF4

Property Value
BioDare ID 12962296599986
Author Aurora Pinas-Fernandez
Institution University of Edinburgh
License CC_BY

Description

Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.

Purpose

Experiment carry out to reparameterize the clock off Succrose

Comments

Sample time 24h. 24h ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Flis Anna

Study: Flis et al. (2015)

Q-PCR LD (12:12) 17C CAB2

Property Value
BioDare ID 13228354371807
Author Aurora Pinas-Fernandez
Institution University of Edinburgh
License CC_BY

Description

Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.

Purpose

Experiment carry out to reparameterize the clock off Succrose

Comments

Sample time 24h. 24h ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Flis Anna

Study: Flis et al. (2015)

Q-PCR LD (12:12) 17C LUX

Property Value
BioDare ID 12820610913763
Author Aurora Pinas-Fernandez
Institution University of Edinburgh
License CC_BY

Description

Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.

Purpose

Experiment carry out to reparameterize the clock off Succrose

Comments

Sample time 24h. 24h ...

Q-PCR LD (12:12) 17C GI

Property Value
BioDare ID 12820606741450
Author Aurora Pinas-Fernandez
Institution University of Edinburgh
License CC_BY

Description

Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.

Purpose

Experiment carry out to reparameterize the clock off Succrose

Comments

Sample time 24h. 24h ...

Q-PCR LD (12:12) 17C TOC1

Property Value
BioDare ID 12820611587928
Author Aurora Pinas-Fernandez
Institution University of Edinburgh
License CC_BY

Description

Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.

Purpose

Experiment carry out to reparameterize the clock off Succrose

Comments

Sample time 24h. 24h ...

Q-PCR LD (12:12) 17C PRR5

Property Value
BioDare ID 12820611188065
Author Aurora Pinas-Fernandez
Institution University of Edinburgh
License CC_BY

Description

Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.

Purpose

Experiment carry out to reparameterize the clock off Succrose

Comments

Sample time 24h. 24h ...

Q-PCR LD (12:12) 17C PRR7

Property Value
BioDare ID 12820611319996
Author Aurora Pinas-Fernandez
Institution University of Edinburgh
License CC_BY

Description

Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.

Purpose

Experiment carry out to reparameterize the clock off Succrose

Comments

Sample time 24h. 24h ...

Q-PCR LD (12:12) 17C PRR9

Property Value
BioDare ID 12820610743262
Author Aurora Pinas-Fernandez
Institution University of Edinburgh
License CC_BY

Description

Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.

Purpose

Experiment carry out to reparameterize the clock off Succrose

Comments

Sample time 24h. 24h ...

Q-PCR LD (12:12) 17C CCA1

Property Value
BioDare ID 12820611467827
Author Aurora Pinas-Fernandez
Institution University of Edinburgh
License CC_BY

Description

Seedlings were entrained for 7 days at 22C in 12:12 white light. Then shifted to 17C 12:12 white light. Samples were collected on the 6th day at 17 degrees.

Purpose

Experiment carry out to reparameterize the clock off Succrose

Comments

Sample time 24h. 24h ...

TiMet WP1.1 WS seedling vs rosette

Property Value
BioDare ID 2843
Author Anna Flis
Institution MPI of Molecular Plant Physiology
License CC_BY

Description

Ws in biological duplicates, from diurnal cycle (12L/12D) with different plants age: 9 and 20 days. Numbers are in transcript copy per cell, obtained assuming 1 g FW contains 25000000 cells.

Purpose

Compare clock important genes transcript levels in seedlings ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Flis Anna

Study: Flis et al. (2015)

TiMet WP1.1 qRT-PCR elf3 LD

Property Value
BioDare ID 2842
Author Anna Flis
Institution MPI of Molecular Plant Physiology
License CC_BY

Description

WT and elf3-4 mutants, in biological duplicates, diurnal light, harvest every 2 hours Numbers are in transcript copy per cell, obtained assuming 1 g FW contains 25000000 cells.

Purpose

TiMet focuses on an important and general biological problem – how are plant ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Flis Anna

Study: Flis et al. (2015)

TiMet WP1.1 qRT-PCR LD to LL and LD

Property Value
BioDare ID 2841
Author Anna Flis
Institution MPI of Molecular Plant Physiology, Golm, Germany
License CC_BY

Description

2 WT (Col, Ws) and 5 clock mutants, in biological duplicates, from three conditions: Diurnal cycle (12L/12D), Extended night (DD), Extended light (LL), harvest every 2 hours. Numbers are in transcript copy per cell, obtained ...

Submitter: Daniel Thedie

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Flis Anna

Study: Flis et al. (2015)

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