Clock genes under Far Red light - 3

Description

Seedlings receive 3hrs of white light to germinate, then are entrained under 12/12 FR/D cycles for 3 days, sprayed, and imaged 24hrs after under 1 FR/D cycle, 3 days of continuous FR and 48h of DD

Purpose

Analysis of the main clock markers under strict FR ...

Analysis of Control Strains Neurospora crassa

Description

Purpose

Compare different siblings of the parental/control strains in the global screening of transcription factors capable to affect the circadian oscilator

Comments

Carbon Source

Description

Purpose

Compare Control Strains in different Carbon Sources

Comments

Entrainment Control Analysis

Description

Purpose

Compare Period Calculations using entrainment and non entrainment strains for 72 hours

Comments

Controls

Description

Purpose

Global Search

Comments

080115H1

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...

080115H2

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

...

210814H1

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x7, Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the ...

210814H2

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x7 light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

Partner experiment: 210814H1

190614H1

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...

190614H2

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

...

010514H1

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...

010514H2

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

...

240414H1

Description

Plants entrained in 12L 12D white light.

Experimental conditions (Red and Blue) 12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3. Dawn is at ZT0 and dusk (shady dark) is at ZT12.

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and ...

240414H2

Description

Experimental conditions (Red and Blue) 12 hours light : 12 hours dark x4 followed by 3 days constant light. light is at 90umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Purpose

Comparison of Light:Shady night versus standard lights ON/OFF to dissect the role of photoreceptors and photosynthesis in the circadian clock.

Comments

...

140515H1

Description

At ZT0 dawn is dim light (6umolm-2s-1) for 3 hours. At ZT3 light steps up to 90 umolm-2s-1. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light (first day with double dawn) for 3 days. ie 12L:12D x4 LLx3.

Purpose

Double dawn experiment based on Virginie and Thiago's design (Stitt Lab).

...

140515H2

Description

ZT0 lights remain switched off until ZT3. At ZT3 light comes on at 90 umolm-2s-1. Dusk is lights off at ZT 12. Dark dawn cycle is for 4 days then continuous light (first day with dark dawn) for 3 days. ie 9L:15D x4 LLx3.

Purpose

Control "dark" dawn experiment for double dawn experiment based on Virginie and Thiago's design ...

280814H1

Description

At ZT0 dawn is dim light (1umolm-2s-1) for 6 hours. At ZT6 light steps up to 90 umolm-2s-2. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light for 4 days. ie 12L:12D x4 LLx4.

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

Pedro is not able to record changes in intensity ...

280814H2

Description

6D: 6L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Partner experiment: 280814H1

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

140814H1

Description

At ZT0 dawn is dim light (1umolm-2s-1) for 6 hours. At ZT6 light steps up to 90 umolm-2s-2. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light for 3 days. ie 12L:12D x4 LLx3.

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

Pedro is not able to record changes in intensity ...

140814H2

Description

6D: 6L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Partner experiment: 140814H1

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

one plate had very bad fungal contamination (plate 2). The samples affected are indicated in the 'experimental conditions'.

070814H1

Description

At ZT0 dawn is dim light (1 umolm-2s-1) for 6 hours. At ZT6 light steps up to 90 umolm-2s-2. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light for 3 days. ie 12L:12D x4 LLx3.

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

Pedro is not able to record changes in intensity ...

070814H2

Description

12L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Partner experiment: 070814H1

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

030714H1

Description

At ZT0 dawn is dim light (6umolm-2s-1) for 6 hours. At ZT6 light steps up to 90 umolm-2s-2. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light for 3 days. ie 12L:12D x4 LLx3.

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

Pedro is not able to record changes in intensity ...

030714H2

Description

12L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Partner experiment: 030714H1

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

100414H1

Description

At ZT0 dawn is dim light (6umolm-2s-1) for 6 hours. At ZT6 light steps up to 90 umolm-2s-2. Dusk is lights off at ZT 12. Double dawn cycle is for 4 days then continuous light for 3 days. ie 12L:12D x4 LLx3.

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

Pedro is not able to record changes in ...

100414H2

Description

12L:12D x4 LLx3. Light intensity is 90 umolm-2s-1 (red = 51 umolm-2s-1, blue = 32 umol-2s-1)

Partner experiment: 100414H1

Purpose

Double dawn experiment to compare to Mark Stitt's results

Comments

270313H1: 21L 7Dx4 LLx3 (T-28)

Description

Plants entrained in 21L 7D Experimental conditions: 21L7Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

5mins of light at the end of daytime during the first three imaging days appear to be absent, ...

130313H1: 14L14D x 4 LL x 3 (T-28)

Description

Plants entrained in 14L 14D Experimental conditions: 14L14Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

Wrong imaging script was used (90mins) for T-28. 5mins, 10mins and 10mins of L was cut out ...

200313H2: 7L 21Dx4 LLx3 (T-28)

Description

Plants entrained in 7L 21D Experimental conditions: 7L21Dx4 LLx3

Purpose

To test whether cca1/lhy double mutant can adjust to change in photoperiod in T-cycle

Comments

5mins of light at the end of daytime on imaging day 2 and day 3 appeared to be absent, using the ...