Short Name: T22_SxPv2 Title: Sexy Plant version 2: inducible and regulable SxP versions Description: The purpose of this investigation is to optimize the SxP version 1, with a switchable expression and increased compound release. Phenodata: ./phenodata_20210920.txt Principal investigator: Diego Orzaez License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes Institutions involved: Instituto de Biología Molecular y Celular de Plantas (IBMCP), Spain; Institute of Agrochemistry and Food Technology (IATA), Spain; The Earlham Institute (EI), UK; National institute of Biology (NIB), Slovenia; Technische Universität Darmstadt (TUDA), Germany; Ecologia y Proteción Agrícola (EPA), Spain Project start date: 1.4.2018 Project end date: 31.3.2021
Investigation position:
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Created: 12th Apr 2022 at 14:07
Last updated: 7th Nov 2024 at 10:03
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PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.
Programme: NIBSys
Public web page: http://susphire.info/susphireproject/
Start date: 1st Apr 2018
End date: 30th Sep 2021
Investigation files _I_T22_SxPv2
Snapshots: No snapshots
Short Name: P1_TransgenicStableSxPv2 Title: Generation of Sexy Plant version 2: transgenic plants with the moth sex pheromones biosynthetic pathway genes regulated by synthetic promotors. Description: The purpose of this study is to generate transgenic stable lines of Nicotiana benthamiana plants ready to express the moth sex pheromone biosynthetic pathway in a regulable way. The moth sex pheromones biosynthetic pathway genes are regulated by synthetic promotors, so in a basal state, the plants ...
Submitter: Marko Petek
Investigation: _I_T22_SxPv2
Assays: _A_SxPGuidedPathway-GCMS, _A_SxPNonGuidedPathway-GCMS, _A_SxPdCasEVNonGuidedPathway-GCMS, _S_P1_TransgenicStableSxPv2-files
Snapshots: No snapshots
Files connected to investigation: _I_T22_SxPv2
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T22_SxPv2
Study: Investigation files
Organisms: No organisms
SOPs: No SOPs
Data files: _INVESTIGATION_METADATA.TXT, phenodata_20210920.txt, phenodata_20210920.xlsx
Snapshots: No snapshots
Files connected to study: _S_P1_TransgenicStableSxPv2
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T22_SxPv2
Study: _S_P1_TransgenicStableSxPv2
Short Name: SxPdCasEVNonGuidedPathway-GCMS Assay Class: WET Assay Type: GCMS Title: SxP dCasEV Non Guided Pathway (EV NGP) Description: Generation and analysis of the stable Nicotiana benthamiana transgenic plants with the moth sex pheromone biosynthetic pathway genes regulated by synthetic promotors, the dCasEV system and not provided with the specific guide in stable. The activation of the pathway was performed by agroinfiltration of the specific guide in trans, so the system is complete and ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T22_SxPv2
Study: _S_P1_TransgenicStableSxPv2
Organisms: No organisms
SOPs: No SOPs
Data files: _ASSAY_METADATA.TXT, output/Quantifications_EV_NGP_Nbenth.xlsx, reports/Short_Report_EV_NGP.txt
Snapshots: No snapshots
Short Name: SxPGuidedPathway-GCMS Assay Class: WET Assay Type: GCMS Title: SxP Guided Pathway (GP) Description: Generation and analysis of the stable Nicotiana benthamiana transgenic plants with the moth sex pheromone biosynthetic pathway genes regulated by synthetic promotors and provided with the specific guide in stable. The purpose of this assay is to measure the volatile moth sex pheromone production by HSPME GCMS of the generated stable Nicotiana benthamiana plants. The activation of the ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T22_SxPv2
Study: _S_P1_TransgenicStableSxPv2
Organisms: No organisms
SOPs: No SOPs
Data files: _ASSAY_METADATA.TXT, output/Quantifications_GP_Nbenth.xlsx, reports/Short_Report_GP.txt
Snapshots: No snapshots
Short Name: SxPNonGuidedPathway-GCMS Assay Class: WET Assay Type: GCMS Title: SxP Non Guided Pathway (NGP) Description: Generation and analysis of the stable Nicotiana benthamiana transgenic plants with the moth sex pheromone biosynthetic pathway genes regulated by synthetic promotors and not provided with the specific guide in stable. The activation of the pathway was performed by agroinfiltration of the dCasEV and the specific guide in trans, so the system is complete and the production of the ...
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T22_SxPv2
Study: _S_P1_TransgenicStableSxPv2
Organisms: No organisms
SOPs: No SOPs
Data files: _ASSAY_METADATA.TXT, output/Quantifications_NGP_Nbenth.xlsx, reports/Short_Report_NGP.txt
Snapshots: No snapshots
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPNonGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T22_SxPv2
Studies: _S_P1_TransgenicStableSxPv2
Assays: _A_SxPNonGuidedPathway-GCMS
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPNonGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T22_SxPv2
Studies: _S_P1_TransgenicStableSxPv2
Assays: _A_SxPNonGuidedPathway-GCMS
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPNonGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T22_SxPv2
Studies: _S_P1_TransgenicStableSxPv2
Assays: _A_SxPNonGuidedPathway-GCMS
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPdCasEVNonGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T22_SxPv2
Studies: _S_P1_TransgenicStableSxPv2
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T22_SxPv2/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T22_SxPv2/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T22_SxPv2
Studies: _S_P1_TransgenicStableSxPv2
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPdCasEVNonGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T22_SxPv2
Studies: _S_P1_TransgenicStableSxPv2
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPdCasEVNonGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T22_SxPv2
Studies: _S_P1_TransgenicStableSxPv2
_p_SUSPHIRE/_I_T22_SxPv2/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T22_SxPv2
Studies: _S_P1_TransgenicStableSxPv2
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPdCasEVNonGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T22_SxPv2
Studies: _S_P1_TransgenicStableSxPv2
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
_p_SUSPHIRE/_I_T22_SxPv2/_S_P1_TransgenicStableSxPv2/_A_SxPNonGuidedPathway-GCMS/
Creator: Marko Petek
Submitter: Marko Petek
Investigations: _I_T22_SxPv2
Studies: _S_P1_TransgenicStableSxPv2
Assays: _A_SxPNonGuidedPathway-GCMS