_I_T12_CuInducible

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Short Name: T12_CuInducible Title: Production and testing of copper inducible dCas9EV system for moth pheromone production Description: dCas9EV system used to activate the synthetic promoters made in T11 was coupled to a copper-inducible promoter made of CBS repeats and a minimal DFR promoter. Such system will be induced via CUP2 protein, which binds to CBS motif in presence of copper. This cascade-like system was tested for production of the moth pheromone pathway. Phenodata: ./phenodata_20210107.txt Principal investigator: Diego Orzaez License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes

SEEK ID: https://fairdomhub.org/investigations/507

Projects: SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Contr...

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Creators and Submitter

Creator

Marko Petek

Submitter

Marko Petek

Activity

Views: 18

Created: 10th Jan 2022 at 11:11

Last updated: 7th Nov 2024 at 10:03

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People (1)
Programmes (1)
Projects (1)
Studies (2)
Assays (6)
Data files (15)
Documents (5)

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Marko Petek

Projects: pISA-tree, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL

Institutions: National Institute of Biology

https://orcid.org/0000-0003-3644-7827

Expertise: Molecular Biology, Transcriptomics, Solanum tuberosum, Plant biology, plant pathways, plant metabolism, Systems Biology

Tools: Molecular biology techniques (RNA/DNA/Protein), Microarray analysis, Bioinformatics, R, RNAseq, RNA interference, metabolic network visualization, metagenomics

PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology

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NIBSys

National Institute of Biology, Department of Biotechnology and Systems Biology projects

Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground

Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology

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SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture

SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.

Programme: NIBSys

Public web page: http://susphire.info/susphireproject/

Start date: 1st Apr 2018

End date: 30th Sep 2021

Organisms: Planococcus citri, Nicotiana benthamiana, Penicillium chrysogenum

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Investigation files

SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture

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Marko Petek

Investigation files _I_T12_CuInducible

Submitter: Marko Petek

Investigation: _I_T12_CuInducible

Assays: _I_T12_CuInducible-files

Snapshots: No snapshots

Created: 10th Jan 2022 at 11:11, Last updated: 7th Nov 2024 at 10:03

_S_P1_TransientExp_Nb

SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture

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Marko Petek

Short Name: P1_TransientExp_Nb Title: Agroinfiltration of copper inducible system in Nicotiana benthamiana Description: The copper inducible dCasEV was tested transiently in Nicotiana benthamiana leaves together with the moth pheromone pathway under the regulation of the synthetic promoters created in T11 Raw Data: https://zenodo.org/communities/susphire/search?page=1&size=20 pISA Study creation date: 2020-10-28 pISA Study creator: ElenaMG Principal investigator: Diego Orzaez License: Creative ...

Submitter: Marko Petek

Investigation: _I_T12_CuInducible

Assays: _A_AgroinfFinalConstructsdCas9EV-GCMS, _A_AgroinfGuidedPathwaywithConstitutivedCas9EV-GCMS, _A_AgroinfNonGuidedPathwaywithCopperInducibledCas9EV-GCMS, _A_AgroinfNonGuidedPathwayywithConstitutivedCas9EV-GCMS, _S_P1_TransientExp_Nb-files

Snapshots: No snapshots

Created: 10th Jan 2022 at 11:11, Last updated: 7th Nov 2024 at 10:03

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_I_T12_CuInducible-files

SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture

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Marko Petek

Files connected to investigation: _I_T12_CuInducible

Submitter: Marko Petek

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: _I_T12_CuInducible

Study: Investigation files

Organisms: No organisms

SOPs: No SOPs

Data files: _INVESTIGATION_METADATA.TXT, phenodata_20210107.txt

Snapshots: No snapshots

Created: 10th Jan 2022 at 11:11, Last updated: 7th Nov 2024 at 10:03

_S_P1_TransientExp_Nb-files

SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture

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Marko Petek

Files connected to study: _S_P1_TransientExp_Nb

Submitter: Marko Petek

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: _I_T12_CuInducible

Study: _S_P1_TransientExp_Nb

Organisms: No organisms

SOPs: No SOPs

Data files: _STUDY_METADATA.TXT

Snapshots: No snapshots

Created: 10th Jan 2022 at 11:12, Last updated: 7th Nov 2024 at 10:03

_A_AgroinfFinalConstructsdCas9EV-GCMS

SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture

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Marko Petek

Short Name: AgroinfFinalConstructsdCas9EV-GCMS Assay Class: WET Assay Type: GCMS Title: Agroinfiltration of final constructs for copper-inducible pathway in Nicotiana benthamiana leaves Description: Two constructs were made containing the three genes of the moth pheromone pathway under the regulation of synthetic promoters made in T11, nptII selection marker and with/without the gRNA1 for activation. In parallel, another construct containing the copper-inducible dCas9EV system with/without the ...

Submitter: Marko Petek

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: _I_T12_CuInducible

Study: _S_P1_TransientExp_Nb

Organisms: No organisms

SOPs: No SOPs

Data files: _ASSAY_METADATA.TXT, output/AgroinfQuants 03-05-21.xlsx, reports/AgroinfGraphs 03-05-21.xlsx

Snapshots: No snapshots

Created: 10th Jan 2022 at 11:12, Last updated: 7th Nov 2024 at 10:03

_A_AgroinfGuidedPathwaywithConstitutivedCas9EV-GCMS

SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture

All creators

Marko Petek

Short Name: AgroinfGuidedPathwaywithConstitutivedCas9EV-GCMS Assay Class: WET Assay Type: GCMS Title: Agroinfiltration of guided pathway for moth pheromones production with constitutive dCas9EV system in Nicotiana benthamiana leaves Description: Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. In the constructs it was also included the guideRNA1 and nptII ...

Submitter: Marko Petek

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: _I_T12_CuInducible

Study: _S_P1_TransientExp_Nb

Organisms: No organisms

SOPs: No SOPs

Data files: _ASSAY_METADATA.TXT, output/agroinfiltrationQuants 07-01-21.xlsx, reports/agroinfiltrationGraphs 07-01-21.xlsx

Snapshots: No snapshots

Created: 10th Jan 2022 at 11:12, Last updated: 7th Nov 2024 at 10:03

_A_AgroinfNonGuidedPathwaywithCopperInducibledCas9EV-GCMS

SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture

All creators

Marko Petek

Short Name: AgroinfNonGuidedPathwaywithCopperInducibledCas9EV-GCMS Assay Class: WET Assay Type: GCMS Title: Agroinfiltration of non-guided pathway for moth pheromones production with copper-inducible dCas9EV system in Nicotiana benthamiana leaves Description: Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. The constructs were first tested transiently in ...

Submitter: Marko Petek

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: _I_T12_CuInducible

Study: _S_P1_TransientExp_Nb

Organisms: No organisms

SOPs: No SOPs

Data files: _ASSAY_METADATA.TXT, output/agroinfQuants(copper)26-01-21.xlsx, reports/agroinfGraphs(copper)26-01-21.xlsx

Snapshots: No snapshots

Created: 10th Jan 2022 at 11:12, Last updated: 7th Nov 2024 at 10:03

_A_AgroinfNonGuidedPathwayywithConstitutivedCas9EV-GCMS

SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture

All creators

Marko Petek

Short Name: AgroinfNonGuidedPathwayywithConstitutivedCas9EV-GCMS Assay Class: WET Assay Type: GCMS Title: Agroinfiltration of nonguided pathway for moth pheromones production with constitutive dCAs9EV system in Nicotiana benthamiana leaves Description: Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. The constructs were first tested transiently in Nicotiana ...

Submitter: Marko Petek

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: _I_T12_CuInducible

Study: _S_P1_TransientExp_Nb

Organisms: No organisms

SOPs: No SOPs

Data files: _ASSAY_METADATA.TXT, output/agroinfQuants26-01-21.xlsx, reports/agroinfGraphs26-01-21.xlsx

Snapshots: No snapshots

Created: 10th Jan 2022 at 11:12, Last updated: 7th Nov 2024 at 10:03

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