BioDare_metadata
Version 1

Original BioDare metadata, converted to json format

SEEK ID: https://fairdomhub.org/data_files/9066?version=1

Filename: BioDare_metadata.json  Download

Format: JSON document

Size: 157 KB

{
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  "status": "in progress",
  "provenance": {
    "desc_version": "1",
    "data_version": "1",
    "uploaded": {
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      "date": "2014-08-06T12:26:24"
    },
    "modified": [
      {
        "by": "SYSTEM USER",
        "date": "2014-09-15T16:54:17"
      },
      {
        "by": "SYSTEM USER",
        "date": "2014-09-08T18:30:46"
      },
      {
        "by": "Yvette Wilson",
        "date": "2014-08-06T12:28:33"
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      {
        "by": "Yvette Wilson",
        "date": "2014-08-06T12:28:23"
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      {
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        "date": "2014-08-06T12:27:46"
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    "owner": "ywilson2",
    "supervisor": "andrew",
    "allowedToRead": [
      "edinburgh",
      "millar",
      "robust",
      "timet"
    ],
    "allowedToWrite": [
      "edinburgh",
      "millar"
    ]
  },
  "hasTSData": "true",
  "desc": {
    "experiment_type": "Luciferase exp",
    "id": "1642",
    "status": "in progress",
    "name": "190614H1",
    "purpose": "Comparison of Light:Shady night versus standard lights ON/OFF to  dissect the role of photoreceptors and photosynthesis in the circadian clock",
    "description": "Plants entrained in 12L 12D white light.\n\nExperimental conditions (Red and Blue)\n12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3.\nDawn is at ZT0 and dusk (shady dark) is at ZT12.",
    "comments": "Pedro is not able to record changes in intensity during an experiment, so please refer to the description & experimental conditions comment for true experimental conditions.\n\nPartner Experiment: 190614H2\n\nImages binned 2x2 to obtain signal from prr7prr9 CCR2:LUC line",
    "provenance": {
      "site": "Edinburgh",
      "author": "Yvette Wilson",
      "start_date": "2014-06-18"
    },
    "growth_condition": {
      "name": "12L:12D",
      "medium": "Agar 1.2% MS 0.5",
      "incubation": "7 days at 4C",
      "growth_space": "Percival",
      "duration": "6.0",
      "comment": "Plants were entrained at light intensity 90 umolm-2s-1 to correspond to the light intensity of the experiment.",
      "temperature_conditions": {
        "label": "const. 22.0C",
        "env_conditions": {
          "type": "constant_temp",
          "label": "const. 22.0C",
          "duration": "6",
          "env_day": {
            "type": "constant_temp",
            "label": "constant temperature",
            "duration": "6",
            "cycle_length": "24",
            "base_value": "22.0",
            "second_value": "22.0"
          }
        }
      },
      "light_conditions": {
        "label": "W: LD 12:00:12:00",
        "light_channel": {
          "spectrum": "white",
          "intensity": "90",
          "source": "tube",
          "env_conditions": {
            "type": "diurnal_light",
            "label": "LD 12:00:12:00",
            "duration": "6",
            "env_day": {
              "type": "diurnal_light",
              "label": "diurnal light",
              "duration": "6",
              "cycle_length": "24",
              "base_value": "0.0",
              "second_value": "100.0",
              "env_period": {
                "start_time": "0:00",
                "duration": "12:00"
              }
            }
          }
        }
      }
    },
    "experimental_condition": {
      "name": "12L:12ShDx4 LLx3",
      "medium": "Agar 1.2% MS 0.5",
      "growth_space": "Percival + LED",
      "duration": "7.0",
      "comment": "12 hours of bright light (90 umolm-2s-2) followed by 12 hours of 'shady dark' (6 umolm-2s-2) for four days and then 3 days of continuous light.\n\nThe first dawn starts at ZT0\n\nThe light intensities are controlled by the arduino.\n\nAt max light intensity red = 51 umolm-2s-1 and blue light intensity is 32 umol-2s-1.\n\nplants were transferred to experimental condiditons at ZT3",
      "temperature_conditions": {
        "label": "const. 22.0C",
        "env_conditions": {
          "type": "constant_temp",
          "label": "const. 22.0C",
          "duration": "7",
          "env_day": {
            "type": "constant_temp",
            "label": "constant temperature",
            "duration": "7",
            "cycle_length": "24",
            "base_value": "22.0",
            "second_value": "22.0"
          }
        }
      },
      "light_conditions": {
        "label": "B+R: 4*LD 12:00:12:00; 3*LL",
        "light_channel": [
          {
            "spectrum": "blue",
            "intensity": "32",
            "source": "LED",
            "env_conditions": {
              "type": "light_complex",
              "label": "4*LD 12:00:12:00; 3*LL",
              "duration": "7",
              "env_day": [
                {
                  "type": "diurnal_light",
                  "label": "diurnal light",
                  "duration": "4",
                  "cycle_length": "24",
                  "base_value": "0.0",
                  "second_value": "100.0",
                  "env_period": {
                    "start_time": "0:00",
                    "duration": "12:00"
                  }
                },
                {
                  "type": "constant_light",
                  "label": "constant light",
                  "duration": "3",
                  "cycle_length": "24",
                  "base_value": "0.0",
                  "second_value": "100.0",
                  "env_period": {
                    "start_time": "0:00",
                    "duration": "24:00"
                  }
                }
              ]
            }
          },
          {
            "spectrum": "red",
            "intensity": "51",
            "source": "LED",
            "env_conditions": {
              "type": "light_complex",
              "label": "4*LD 12:00:12:00; 3*LL",
              "duration": "7",
              "env_day": [
                {
                  "type": "diurnal_light",
                  "label": "diurnal light",
                  "duration": "4",
                  "cycle_length": "24",
                  "base_value": "0.0",
                  "second_value": "100.0",
                  "env_period": {
                    "start_time": "0:00",
                    "duration": "12:00"
                  }
                },
                {
                  "type": "constant_light",
                  "label": "constant light",
                  "duration": "3",
                  "cycle_length": "24",
                  "base_value": "0.0",
                  "second_value": "100.0",
                  "env_period": {
                    "start_time": "0:00",
                    "duration": "24:00"
                  }
                }
              ]
            }
          }
        ]
      }
    },
    "measurement": {
      "technique": "Luciferase luminescence",
      "equipment": "HAM 1",
      "parameters": "Imaging script started 1 hr 25 minutes after ZT0\n55mins wait, 5mins lights off, 30mins capture",
      "description": "Seedlings were treated on 6th day with Luciferin. They were then transferred Experimental conditions. Imaging was started on d7."
    },
    "sample_preparation": {
      "method": "Plants sprayed with luciferin on day 6 of growth conditions and transferred to  experimental conditions. Imaging was started on day 7."
    },
    "samples": {
      "sample": [
        {
          "sample_id": "1",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "Img Background",
            "line": "unknown",
            "provenance": "Robust stock"
          },
          "marker": "Actin",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "2",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "3",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "4",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "5",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "6",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "7",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
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          "ignored": "false",
          "param": {
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            "@value": "Luc"
          }
        },
        {
          "sample_id": "8",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
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            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "9",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
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          "ignored": "false",
          "param": {
            "@name": "TAG",
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          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "11",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
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          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
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          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
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          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
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            "provenance": "Robust stock"
          },
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          "ignored": "false",
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            "@name": "TAG",
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          "sample_type": "seedling",
          "origin": "seedling",
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          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
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            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
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          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
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          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
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            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "15",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
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          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
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          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
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            "genotype": "Wild Type",
            "line": "M0008",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "89",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "90",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "91",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "92",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "93",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "94",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "95",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "96",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "97",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "98",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "99",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "100",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "elf3-4",
            "line": "M0026",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "101",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "102",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "103",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "104",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "105",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "106",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "107",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "108",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "109",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "110",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "111",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "112",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Wasilewski (WS)",
            "genotype": "Wild Type",
            "line": "M0007",
            "provenance": "Robust stock"
          },
          "marker": "CCA1",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "ignored": "false",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        }
      ]
    }
  },
  "pedro": {
    "@xmlns:xsi": "http://www.w3.org/2001/XMLSchema-instance",
    "@xsi:schemaLocation": "/W:/communal/DataDescription/models/luc/model/newluc_model.xsd",
    "id": "will be updated by the system",
    "status": "in progress",
    "name": "190614H1",
    "purpose": "Comparison of Light:Shady night versus standard lights ON/OFF to  dissect the role of photoreceptors and photosynthesis in the circadian clock",
    "description": "Plants entrained in 12L 12D white light.\n\nExperimental conditions (Red and Blue)\n12 hours light (90umolm-2s-1): 12 hours shady dark (6 umolm-2s-1) x4, constant light x3.\nDawn is at ZT0 and dusk (shady dark) is at ZT12.",
    "comments": "Pedro is not able to record changes in intensity during an experiment, so please refer to the description & experimental conditions comment for true experimental conditions.\n\nPartner Experiment: 190614H2\n\nImages binned 2x2 to obtain signal from prr7prr9 CCR2:LUC line",
    "provenance": {
      "site": "Edinburgh",
      "author": "Yvette Wilson",
      "start_date": "2014-06-18"
    },
    "growth_condition": {
      "name": "12L:12D",
      "medium": "Agar 1.2% MS 0.5",
      "incubation": "7 days at 4C",
      "growth_space": "Percival",
      "duration": "6",
      "comment": "Plants were entrained at light intensity 90 umolm-2s-1 to correspond to the light intensity of the experiment.",
      "temperature_conditions": {
        "constant_temp": {
          "cycle_length": "24",
          "base_temp": "22"
        }
      },
      "light_conditions": {
        "light_channel": {
          "spectrum": "white",
          "intensity": "90",
          "source": "tube",
          "diurnal_light": {
            "cycle_length": "24",
            "start_time": "0",
            "duration": "12"
          }
        }
      }
    },
    "experimental_condition": {
      "name": "12L:12ShDx4 LLx3",
      "medium": "Agar 1.2% MS 0.5",
      "growth_space": "Percival + LED",
      "duration": "7",
      "comment": "12 hours of bright light (90 umolm-2s-2) followed by 12 hours of 'shady dark' (6 umolm-2s-2) for four days and then 3 days of continuous light.\n\nThe first dawn starts at ZT0\n\nThe light intensities are controlled by the arduino.\n\nAt max light intensity red = 51 umolm-2s-1 and blue light intensity is 32 umol-2s-1.\n\nplants were transferred to experimental condiditons at ZT3",
      "temperature_conditions": {
        "constant_temp": {
          "cycle_length": "24",
          "base_temp": "22"
        }
      },
      "light_conditions": {
        "light_channel": [
          {
            "spectrum": "blue",
            "intensity": "32",
            "source": "LED",
            "light_complex_settings": {
              "light_days": [
                {
                  "start_day": "1",
                  "duration": "4",
                  "diurnal_light": {
                    "cycle_length": "24",
                    "start_time": "0",
                    "duration": "12"
                  }
                },
                {
                  "start_day": "5",
                  "duration": "3",
                  "constant_light": {
                    "cycle_length": "24"
                  }
                }
              ]
            }
          },
          {
            "spectrum": "red",
            "intensity": "51",
            "source": "LED",
            "light_complex_settings": {
              "light_days": [
                {
                  "start_day": "1",
                  "duration": "4",
                  "diurnal_light": {
                    "cycle_length": "24",
                    "start_time": "0",
                    "duration": "12"
                  }
                },
                {
                  "start_day": "5",
                  "duration": "3",
                  "constant_light": {
                    "cycle_length": "24"
                  }
                }
              ]
            }
          }
        ]
      }
    },
    "measurement": {
      "technique": "Luciferase luminescence",
      "equipment": "HAM 1",
      "parameters": "Imaging script started 1 hr 25 minutes after ZT0\n55mins wait, 5mins lights off, 30mins capture",
      "description": "Seedlings were treated on 6th day with Luciferin. They were then transferred Experimental conditions. Imaging was started on d7."
    },
    "sample_preparation": {
      "method": "Plants sprayed with luciferin on day 6 of growth conditions and transferred to  experimental conditions. Imaging was started on day 7."
    },
    "samples": {
      "sample": [
        {
          "sample_id": "1",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "Img Background",
            "provenance": "Robust stock"
          },
          "marker": "Actin",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "2",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "3",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "4",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
        },
        {
          "sample_id": "5",
          "sample_type": "seedling",
          "origin": "seedling",
          "growth_stage": "seedling",
          "age": "7",
          "genetic_info": {
            "species": "Arabidopsis thaliana",
            "background": "Columbia (Col)",
            "genotype": "prr7-3 prr9-1",
            "line": "G0011",
            "provenance": "Robust stock"
          },
          "marker": "CCR2",
          "growth_cond_name": "12L:12D",
          "experiment_cond_name": "12L:12ShDx4 LLx3",
          "param": {
            "@name": "TAG",
            "@value": "Luc"
          }
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}
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Yvette Wilson

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Views: 50   Downloads: 3

Created: 18th Jun 2026 at 15:05

Last updated: 18th Jun 2026 at 15:05

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Version 1 (earliest) Created 18th Jun 2026 at 15:05 by Daniel Thedie

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