Assays

Training of the model, parameter estimation using Evolutionary Programming using metabolomics, proteomics and some flux data.

Protein copy number at 6h, 12h, 24h, 48h, 72h, 96h, average values and SD for the measurements

Metabolomics time series measurements for internal metabolites for 6h, 24h and 48h for multiple experiments. Largely based on MAss spectrometry, bioluminescence kits to measure NAD, NADH at 24h, other time points are infered from relative measurements times the absolute measurements at 24h.

Measurements of external metabolites based on growth curve data. Flux estimates for uptake of external metabolites such as glucose and production rates for external metabolites lactate and acetate