Assays
What is an Assay?Training of the model, parameter estimation using Evolutionary Programming using metabolomics, proteomics and some flux data.
Submitter: Niels Zondervan
Biological problem addressed: Model Analysis Type
Investigation: Modelling of M. pneumoniae metabolism
Study: Core Model training
Protein copy number at 6h, 12h, 24h, 48h, 72h, 96h, average values and SD for the measurements
Submitter: Niels Zondervan
Assay type: Proteomics
Technology type: Technology Type
Investigation: Modelling of M. pneumoniae metabolism
Study: Proteomics analysis
Metabolomics time series measurements for internal metabolites for 6h, 24h and 48h for multiple experiments. Largely based on MAss spectrometry, bioluminescence kits to measure NAD, NADH at 24h, other time points are infered from relative measurements times the absolute measurements at 24h.
Submitter: Niels Zondervan
Assay type: Experimental Assay Type
Technology type: Mass Spectrometry
Investigation: Modelling of M. pneumoniae metabolism
Study: Metabolomics measurements
Measurements of external metabolites based on growth curve data. Flux estimates for uptake of external metabolites such as glucose and production rates for external metabolites lactate and acetate
Submitter: Niels Zondervan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Modelling of M. pneumoniae metabolism
Study: Metabolomics measurements