Upcycling of hydrolyzed PET by microbial conversion to a fatty acid derivative

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Abstract:

The enzymatic degradation of polyethylene terephthalate (PET) results in a hydrolysate consisting almost exclusively of its two monomers, ethylene glycol and terephthalate. To biologically valorize the PET hydrolysate, microbial upcycling into high-value products is proposed. Fatty acid derivatives hydroxyalkanoyloxy alkanoates (HAAs) represent such valuable target molecules. HAAs exhibit surface-active properties and can be exploited in the catalytical conversion to drop-in biofuels as well as in the polymerization to bio-based poly(amide urethane). This chapter presents the genetic engineering methods of pseudomonads for the metabolization of PET monomers and the biosynthesis of HAAs with detailed protocols concerning product purification.

SEEK ID: https://fairdomhub.org/publications/587

DOI: 10.1016/bs.mie.2020.12.025

Projects: MIX-UP

Publication type: Book

Journal: Methods in Enzymology

Book Title: Methods in Enzymology

Publisher: Elsevier

Citation: Methods in Enzymology,Elsevier

Date Published: 2021

Registered Mode: by DOI

Authors: Gina Welsing, Birger Wolter, Henric M.T. Hintzen, Till Tiso, Lars M. Blank

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Birger Wolter

Citation

Welsing, G., Wolter, B., Hintzen, H. M. T., Tiso, T., & Blank, L. M. (2021). Upcycling of hydrolyzed PET by microbial conversion to a fatty acid derivative. In Methods in Enzymology (pp. 391–421). Elsevier. https://doi.org/10.1016/bs.mie.2020.12.025

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Created: 9th Feb 2021 at 13:25

Last updated: 8th Dec 2022 at 17:26

Upcycling of hydrolyzed PET by microbial conversion to a fatty acid derivative

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