Functional analysis of new transporters involved in stress tolerance inPseudomonas putidaDOT-T1E

Abstract:

Pseudomonas putida DOT-T1E is a highly solvent-tolerant strain. Although the main mechanism that confers solvent tolerance to the strain is the TtgGHI efflux pump, a number of other proteins are also involved in the response to toluene. Previous proteomic and transcriptomic analysis carried out in our lab with P. putida DOT-T1E, and the solvent-sensitive strain, P. putida KT2440, revealed several transporters that were induced in the presence of toluene. We prepared five mutants of the corresponding genes in P. putida DOT-T1E and analysed their phenotypes with respect to solvent tolerance, stress endurance and growth with different carbon, nitrogen and sulfur sources. The data clearly demonstrated that two transporters (Ttg2ABC and TtgK) are involved in multidrug resistance and toluene tolerance, whereas another (homologous to PP0219 of P. putida KT2440) is a sulfate/sulfite transporter. No clear function could be assigned to the other two transporters. Of the transporters shown to be involved in toluene tolerance, one (ttg2ABC) belongs to the ATP-Binding Cassette (ABC) family, and is involved in multidrug resistance in P. putida DOT-T1E, while the other belongs to the Major Facilitator Superfamily and exhibits homology to a putative transporter of the Bcr/CflA family that has not previously been reported to be involved in toluene tolerance.

SEEK ID: https://fairdomhub.org/publications/4

DOI: 10.1111/j.1758-2229.2009.00093.x

Projects: PSYSMO

Publication type: Not specified

Citation:

Date Published: 1st Nov 2009

Registered Mode: Not specified

Authors: Vanina García, Patricia Godoy, Craig Daniels, Ana Hurtado, , Ana Segura

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Citation
García, V., Godoy, P., Daniels, C., Hurtado, A., Ramos, J. L., & Segura, A. (2010). Functional analysis of new transporters involved in stress tolerance in Pseudomonas putida DOT‐T1E. In Environmental Microbiology Reports (Vol. 2, Issue 3, pp. 389–395). Wiley. https://doi.org/10.1111/j.1758-2229.2009.00093.x
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Created: 6th May 2010 at 08:26

Last updated: 8th Dec 2022 at 17:25

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