SOPs

Assay methodologies for individual glycolytic isoenzymes from the Mendes Group, University of Manchester, UK

Creator: Hanan Messiha

Contributor: Walter Glaser

Sample preparation - SOP for sampling, preparation of cell-free extracts, and determination of total extracted protein

Creator: Femke Mensonides

Contributor: Maksim Zakhartsev

General protocol for measuring the kinetic parameters of the purified glycolytic enzymes from Saccharomyces cerevisiae - SOP for measuring the kinetic parameters of the purified glycolytic isoenzymes

Creator: Hanan Messiha

Contributor: Maksim Zakhartsev

This is the general protocol for the glycolytic enzyme measurements. Detailed Information for each Enzyme can be found in the SOP: Assays for measuring the activities of the individual glycolytic isoenzymes of Saccharomyces cerevisiae

Creator: Hanan Messiha

Contributor: Walter Glaser

This protocol is designed to prepare sufficient amounts of high-quality total
RNA from the yeast saccharomyces cerevisiae grown in liquid culture for
analysis on microarrays.

Creators: Walter Glaser, Christa Gregori

Contributor: Walter Glaser

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