Data files
Strain MK423 was grown under the same conditions as used when growing cells for microscopy analysis; cells of OD600 = 0.4 were diluted 100-fold in C+Y medium with 0.1 mM ZnCl2 and incubated for 2.5 hours until OD600 = 0.15. Cells were then harvested by centrifugation for 5 min at 6500 x g at 4°C. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit (Promega) as described previously (Slager et al. 2014 Cell). Fragmentation was performed using Covaris instrument, and libraries
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Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
Strain MK422 was grown under the same conditions as used when growing cells for microscopy analysis; cells of OD600 = 0.4 were diluted 100-fold in C+Y medium with 0.1 mM ZnCl2 and incubated for 2.5 hours until OD600 = 0.15. Cells were then harvested by centrifugation for 5 min at 6500 x g at 4°C. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit (Promega) as described previously (Slager et al. 2014 Cell). Fragmentation was performed using Covaris instrument, and libraries
...
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
Strain MK350 was grown under the same conditions as used when growing cells for microscopy analysis; cells of OD600 = 0.4 were diluted 100-fold in C+Y medium with 0.1 mM ZnCl2 and incubated for 2.5 hours until OD600 = 0.15. Cells were then harvested by centrifugation for 5 min at 6500 x g at 4°C. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit (Promega) as described previously (Slager et al. 2014 Cell). Fragmentation was performed using Covaris instrument, and libraries
...
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
WT 110901_SN865_B_L006_R1_GQC-28-
ATCACG.fastq.gz
100 19.624.852 1,29
llumina fastq format
4 lines for each sequence:
1- Unique identifier, with the following format:
@::::#/
2- Sequence (A, T, C ,G or N (undetermined) only)
3- Orientation (always forward without mapping)
4- Quality value for each base, corresponding to a Phred-like score encoded in ASCII format, with an
offset of of 33 (e.g. “J” gives a value of 41) and is in accordance with sanger FASTQ format.
The sequence file is compressed
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Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
S. pneumoniae RNA-Seq
Barcodes:
HPUra 1: CAGATC
HPUra 2: ACTTGA
Kanamycin: AGTCAA
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
S. pneumoniae RNA-Seq
Barcodes:
Control 1: CAGATC
Control 2: ACTTGA
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
E. coli RNA-Seq
Barcodes:
Trimethoprim 1: CCGTCC
Trimethoprim 2: GTAGAG
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
E. coli RNA-Seq
Barcodes:
Control 1: AGTTCC
Control 2: ATGTCA
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening
E. coli DNA-Seq
Barcodes:
control 1: GTGGCC
control 2: GTTTCG
Trimethoprim 1: CACTCA
Trimethoprim 2: CAGGCG
Creator: Jan-Willem Veening
Submitter: Jan-Willem Veening