Assays

What is an Assay?

8 Assays visible to you, out of a total of 8

Transcriptome data for chemostat cultivated samples

BaCell-SysMO

Transcriptome analysis for the samples harvested from Chemostat cultivated samples.

Submitter: Praveen kumar Sappa

Assay type: Transcriptomics

Technology type: Microarray

Investigation: Multiomics study of Bacillus subtilis under osm...

Study: B. subtilis_SysMo2_Chemostat_growthrate-salt

Organisms: No organisms

SOPs: RNA isolation and quality check by Agilent bioa..., SOP for cDNA labelling and Microarray hybridizaton

Data files: 1 hidden item

Snapshots: No snapshots

Created: 11th Apr 2011 at 14:38, Last updated: 8th Nov 2017 at 14:21

Absolute quantification of proteins using QconCAT technology

BaCell-SysMO

Absolute quantification of proteins using heavy labeled QconCAT as an internal standard and quantifying the native proteins in the complex sample via scheduled Multiple Reaction Monitoring(MRM) .

Submitter: Praveen kumar Sappa

Assay type: Proteomics

Technology type: Liquid Chromatography Mass Spectrometry

Investigation: Multiomics study of Bacillus subtilis under osm...

Study: B. subtilis_SysMo2_Chemostat_growthrate-salt

Organisms: Bacillus subtilis : 168 (wild-type / wild-type) (chemostat)

SOPs: No SOPs

Data files: 1 hidden item

Snapshots: No snapshots

Created: 21st May 2011 at 15:36, Last updated: 8th Nov 2017 at 14:21

Transcritome data_Identification of targets of the essential RNase Y of Bacillus subtilis

BaCell-SysMO

No description specified

Submitter: Praveen kumar Sappa

Assay type: Transcriptomics

Technology type: Custom Array

Investigation: Identification of targets of the essential RNas...

Study: Identification of targets of the essential RNas...

Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph)

SOPs: RNA isolation and quality check by Agilent bioa..., SOP for cDNA labelling and Microarray hybridizaton

Data files: Trancriptome data - RNA processing in Bacillus ...

Snapshots: No snapshots

Created: 14th Dec 2012 at 18:56, Last updated: 8th Nov 2017 at 14:21

Fermentation-BM5_SysMo

BaCell-SysMO

No description specified

Submitter: Praveen kumar Sappa

Assay type: Glucose Pulse

Technology type: Technology Type

Investigation: The transition from growing to non-growing Baci...

Study: Batchfermentation exp-starv01_090204

Organisms: Bacillus subtilis (batch)

SOPs: 1 hidden item

Data files: Batchfermentation_Exp-Starv01_Fermentationdetai..., batchfermentation exp-starv01 transcriptome_Mic..., batchfermentation exp-starv01 transcriptome_Mic...

Snapshots: No snapshots

Created: 18th May 2010 at 10:08, Last updated: 8th Nov 2017 at 14:21

Tiling array analysis of continuous growth stress conditions in SMM

BaCell-SysMO

B. subtilis was grown in SMM media with glucose as carbon source and the samples for RNA were harvested OD578nm- 1.0). The stress conditions that were applied over here are growthat 57°C, 16°C, 1.2M Nacl and 37°C(control). All the samples were analysed for transcriptome as biological triplicates.

Submitter: Praveen kumar Sappa

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Redefining the Complete Transcriptome of Bacill...

Study: Transcriptome of continuously stressed B. subtilis

Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph)

SOPs: No SOPs

Data files: Continuous growth_16°C_SMM_Growth curve, Continuous growth_57°C_SMM_Growth curve, Contious growth_1.2M NaCl_SMM_Transcritome_Nimb..., Contious growth_16°C_SMM_Transcritome_Nimblegen, Contious growth_57°C_SMM_Transcritome_Nimblegen, Contiuous growth_37°C_SMM(control)_Growth curve., The condition-dependent transcriptome of Bacill...

Snapshots: No snapshots

Created: 18th May 2010 at 12:45, Last updated: 8th Nov 2017 at 14:21

Tiling Array analysis of shocked B. subtilis cells

BaCell-SysMO

No description specified

Submitter: Praveen kumar Sappa

Assay type: Transcriptomics

Technology type: Technology Type

Investigation: Redefining the Complete Transcriptome of Bacill...

Study: Transcriptome of shocked B. subtilis cells

Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph) (batch)

SOPs: No SOPs

Data files: The condition-dependent transcriptome of Bacill...

Snapshots: No snapshots

Created: 18th May 2010 at 13:43, Last updated: 8th Nov 2017 at 14:21

Tiling Array analysis of glucose strarved B. subtilis cells

BaCell-SysMO

B. subtilis was grown in M9 media with glucose as carbon source and the samples were harvested during exponential phase (OD600nm- 0.4), early stationary phase(OD600nm- 1.3), late stationary phase(OD600nm- 1.0). All the samples were analysed for transcriptome as biological triplicates.

Submitter: Praveen kumar Sappa

Assay type: Transcriptomics

Technology type: Microarray

Investigation: Redefining the Complete Transcriptome of Bacill...

Study: Transcriptome analysis of glucose starvation in...

Organisms: Bacillus subtilis : 168 Trp+ (wild-type / tryptophan prototroph) (batch)

SOPs: SOP for cultivation of B.Subtilis, Total RNA isolation from B.Subtilis

Data files: M9_shake_flask_exp_trans_stat_transcriptome_nim..., The condition-dependent transcriptome of Bacill...

Snapshots: No snapshots

Created: 18th May 2010 at 13:47, Last updated: 8th Nov 2017 at 14:21

Gene expression(Transcriptome)

BaCell-SysMO