Analytes_manual-EAG.txt
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_p_SUSPHIRE/_I_T24_phero/_S_P5_EAGresponse/_A_EAG_manual-EAG/

SEEK ID: https://fairdomhub.org/data_files/5631?version=1

Filename: Analytes_manual-EAG.txt

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#Item name	Manual EAG runs to test biological activity of plant produced pheromone
Creation date	20-Nov-21
#Extraction
Extract ID	SxPv1-2
Extract Name	SxPv1-2 fractions
Extraction Method	Solvent extraction with 40 mL toluene (per 10g leaves) with magnetic agitation for 12h. Plant material previously grounded with sand.
Date Extraction	
#Post Extraction
Derivatization or Labelling	none	
Date Derivatization or Labelling	none
Derivatized or labeled Extract ID	none
Derivatized or labeled Extract Name	none
Other Post Extraction Procedures	fractionation of the extract in a gravity column using silical gel as stationary phase and hexane-eter as solvent. Selection of fractions containing biosynthetic Z11C16OL for EAG.
Storage	direct analysis
#Chromatography
Date GC-MS Run	
GC Instrument	none
GC Autosampler Model	none
GC Column model	
GC Column type	
Guard Column	none
#Mass spectrometry
MS Scan polarity	
MS Scan mz range	
MS Instrument	none
MS Ion source	
Mass analyzer	
Raw Spectral Data Files	Blank
#Electroantennography
Instrument	Syntech CS-55 stimulus controller
Air flow	50 mlmin
Stimuli deliverer	Pasteur pipette with filter paper piece
#Other
Operator	Sandra Vacas
Notes

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