meta_AType_SxFmycelia.txt Version 1
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/
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meta_AType_SxFmycelia.txt
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#Item name Pheromone detection in fungi mycelia Lab manager Jaime Primo Sample collection protocol SxF fungi provided by Elena Moreno Extraction protocol Solvent extraction of mycelia with 50mL ethyl acetate for 1h with magentic stirring. Concentration up to 1mL and syringe filtration. Chromatography protocol 1ul injection, oven temperature program: 60C for 1 min, raise at 10C/min up to 110C, mantained for 5 min; then, raise at 10C/min up to 150C, mantained for 3 min, and finally raised at 10C/min up to 300C, hold for 1 min. Mass spectrometry protocol FS mode:39 to 450 uma at 70 eV. SRM mode:82-67 at 5eV, 95-67 at 10eV, 96-54 at 10eV, and 96-81 at 5eV.
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Created: 12th Apr 2022 at 16:00
Last updated: 7th Nov 2024 at 10:04
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Version 1 (earliest) Created 12th Apr 2022 at 16:00 by Marko Petek
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https://orcid.org/0000-0003-3644-7827PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.
Synthetic biology data management spreadsheet: https://fairdomhub.org/data_files/6036
Programme: NIBSys
Public web page: http://susphire.info/susphireproject/
Start date: 1st Apr 2018
End date: 30th Sep 2021
Short Name: T23_SxF Title: Moth pheromone production in filamentous fungi SxF Description: Demonstration of moth pheromone production in filamentous fungi by solvent extractions of liquid cultures and mycelial biomass and GC-MS/MS analysis. Constitutive and inducible expression of moth pheromones in filamentous fungi Penicillium species will be developed using a dCas9-based copper inducible system. An initial proof of concept will also be performed in P. digitatum using constitutive promoters. ...
Submitter: Marko Petek
Studies: Investigation files, _S_P1_ConstExp_Pdigitatum, _S_P5_SxFliquids, _S_P5_SxFmycelia
Assays: _A_FS-GC-MS, _A_FSM-GC-MS, _A_SRM-GC-MS, _A_SRMM-GC-MS, _A_qRTPCRMothPheromonesEnzymes-Q-RT-PCR, _I_T23_SxF-files, _S_P1_ConstExp_Pdigitatum-files, _S_P5_SxFliquids-files, _S_P5_SxFmycelia-files
Short Name: P5_SxFliquids Title: Pheromone detection in liquid extracts of SxF Description: Pheromone detection in liquid extracts of modified filamentous fungi (SxF) by liquid-liquid solvent extraction and GC-MS/MS analysis Raw Data: pISA Study creation date: 21-Sep-22 pISA Study creator: Sandra Vacas Principal investigator: * License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T23_SxF
Assays: _A_FS-GC-MS, _A_SRM-GC-MS, _S_P5_SxFliquids-files
Files connected to study: _S_P5_SxFliquids
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: _S_P5_SxFliquids
