meta_AType_SxFliquids.txt Version 1
_p_SUSPHIRE/_I_T23_SxF/_S_P5_SxFliquids/
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meta_AType_SxFliquids.txt
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#Item name Pheromone detection in fungi liquid culture Lab manager Jaime Primo Sample collection protocol SxF fungi provided by Elena Moreno Extraction protocol Liquid-liquid extraction with 30mL ethyl acetate (x3 times). Concentration up to 1mL and syringe filtration. Chromatography protocol 1ul injection, oven temperature program: 60C for 1 min, raise at 10C/min up to 110C, mantained for 5 min; then, raise at 10C/min up to 150C, mantained for 3 min, and finally raised at 10C/min up to 300C, hold for 1 min. Mass spectrometry protocol FS mode:39 to 450 uma at 70 eV. SRM mode:82-67 at 5eV, 95-67 at 10eV, 96-54 at 10eV, and 96-81 at 5eV.
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Created: 12th Apr 2022 at 16:00
Last updated: 7th Nov 2024 at 10:04
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Version 1 (earliest) Created 12th Apr 2022 at 16:00 by Marko Petek
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https://orcid.org/0000-0003-3644-7827PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.
Synthetic biology data management spreadsheet: https://fairdomhub.org/data_files/6036
Programme: NIBSys
Public web page: http://susphire.info/susphireproject/
Start date: 1st Apr 2018
End date: 30th Sep 2021
Short Name: T23_SxF Title: Moth pheromone production in filamentous fungi SxF Description: Demonstration of moth pheromone production in filamentous fungi by solvent extractions of liquid cultures and mycelial biomass and GC-MS/MS analysis. Constitutive and inducible expression of moth pheromones in filamentous fungi Penicillium species will be developed using a dCas9-based copper inducible system. An initial proof of concept will also be performed in P. digitatum using constitutive promoters. ...
Submitter: Marko Petek
Studies: Investigation files, _S_P1_ConstExp_Pdigitatum, _S_P5_SxFliquids, _S_P5_SxFmycelia
Assays: _A_FS-GC-MS, _A_FSM-GC-MS, _A_SRM-GC-MS, _A_SRMM-GC-MS, _A_qRTPCRMothPheromonesEnzymes-Q-RT-PCR, _I_T23_SxF-files, _S_P1_ConstExp_Pdigitatum-files, _S_P5_SxFliquids-files, _S_P5_SxFmycelia-files
Short Name: P5_SxFliquids Title: Pheromone detection in liquid extracts of SxF Description: Pheromone detection in liquid extracts of modified filamentous fungi (SxF) by liquid-liquid solvent extraction and GC-MS/MS analysis Raw Data: pISA Study creation date: 21-Sep-22 pISA Study creator: Sandra Vacas Principal investigator: * License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T23_SxF
Assays: _A_FS-GC-MS, _A_SRM-GC-MS, _S_P5_SxFliquids-files
Files connected to study: _S_P5_SxFliquids
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: _S_P5_SxFliquids
