phenodata_20211203.txt Version 1
_p_SUSPHIRE/_I_T23_SxF/
SEEK ID: https://fairdomhub.org/data_files/5600?version=1
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phenodata_20211203.txt
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SampleID SampleName SampleDescription Species Strain ProjectPartner Comments FileName Date_of_MS MSmode Operator _A_FS-GC-MS _A_SRM-GC-MS _A_FSM-GC-MS _A_SRMM-GC-MS LD001 SxF3 Sexy-fungi extract line 3 Penicillium chrysogenum Q176_PCEM33431 EPA 210222 SxF 3.raw 22.feb.21 SRM Sandra yes LD002 SxF4 Sexy-fungi extract line 4 Penicillium chrysogenum Q176_PCEM33441 EPA 210222 SxF 4.raw 22.feb.21 SRM Sandra yes LD003 SxF5 Sexy-fungi extract line 5 Penicillium chrysogenum Q176_PCEM33451 EPA 210222 SxF 5.raw 22.feb.21 SRM Sandra yes LD004 SxF6 Sexy-fungi extract line 6 Penicillium chrysogenum Q176_PCEM33461 EPA 210222 SxF 6.raw 22.feb.21 SRM Sandra yes LD005 WT wild type fungi extract Penicillium chrysogenum WT EPA 210222 WTF.raw 22.feb.21 SRM Sandra yes LD006 WTF_FS wild type fungi extract Penicillium chrysogenum WT EPA 210222 WTF_FS.raw 22.feb.21 FS Sandra yes LD007 SxF3_FS Sexy-fungi extract line 3 Penicillium chrysogenum Q176_PCEM33431 EPA 210222 SxF 3_FS.raw 22.feb.21 FS Sandra yes LD008 SxF4_FS Sexy-fungi extract line 4 Penicillium chrysogenum Q176_PCEM33441 EPA 210222 SxF 4_FS.raw 22.feb.21 FS Sandra yes LD009 SxF5_FS Sexy-fungi extract line 5 Penicillium chrysogenum Q176_PCEM33451 EPA 210222 SxF 5_FS.raw 23.feb.21 FS Sandra yes LD010 SxF6_FS Sexy-fungi extract line 6 Penicillium chrysogenum Q176_PCEM33461 EPA 210222 SxF 6_FS.raw 23.feb.21 FS Sandra yes LD011 WT_FS_2 wild type fungi extract Penicillium chrysogenum WT EPA 210906 WT_FS.raw 6.sep.21 FS Sandra yes LD012 SxF3_FS_2 Sexy-fungi extract line 3 Penicillium chrysogenum Q176_PCEM33431 EPA 210906 SxF3_FS.raw 6.sep.21 FS Sandra yes LD013 SxF4_FS_2 Sexy-fungi extract line 4 Penicillium chrysogenum Q176_PCEM33441 EPA 210908 SxF4_FS.raw 8.sep.21 FS Sandra yes LD014 SxF5_FS_2 Sexy-fungi extract line 5 Penicillium chrysogenum Q176_PCEM33451 EPA 210909 SxF5 FS.raw 9.sep.21 FS Sandra yes LD015 WT_2 wild type fungi extract Penicillium chrysogenum WT EPA 210908 WT.raw 8.sep.21 SRM Sandra yes LD016 SxF3_2 Sexy-fungi extract line 3 Penicillium chrysogenum Q176_PCEM33431 EPA 210908 SxF3.raw 8.sep.21 SRM Sandra yes LD017 SxF4_2 Sexy-fungi extract line 4 Penicillium chrysogenum Q176_PCEM33441 EPA 210908 SxF4.raw 8.sep.21 SRM Sandra yes LD018 SxF5_2 Sexy-fungi extract line 5 Penicillium chrysogenum Q176_PCEM33451 EPA 210909 SxF5.raw 9.sep.21 SRM Sandra yes MD001 SxF3_M Sexy-fungi extract line 3 Penicillium chrysogenum Q176_PCEM33431 EPA 210222 SxF 3 M.raw 23.feb.21 SRM Sandra yes MD002 SxF4_M Sexy-fungi extract line 4 Penicillium chrysogenum Q176_PCEM33441 EPA 210222 SxF 4 M.raw 23.feb.21 SRM Sandra yes MD003 SxF5_M Sexy-fungi extract line 5 Penicillium chrysogenum Q176_PCEM33451 EPA 210222 SxF 5 M.raw 23.feb.21 SRM Sandra yes MD004 SxF6_M Sexy-fungi extract line 6 Penicillium chrysogenum Q176_PCEM33461 EPA 210222 SxF 6 M.raw 23.feb.21 SRM Sandra yes MD005 WT_M wild type fungi extract Penicillium chrysogenum WT EPA 210222 WTF M.raw 23.feb.21 SRM Sandra yes MD006 WT_M_FS wild type fungi extract Penicillium chrysogenum WT EPA 210222 WTF_FS M.raw 23.feb.21 FS Sandra yes MD007 SxF3_M_FS Sexy-fungi extract line 3 Penicillium chrysogenum Q176_PCEM33431 EPA 210222 SxF 3_FS M.raw 23.feb.21 FS Sandra yes MD008 SxF4_M_FS Sexy-fungi extract line 4 Penicillium chrysogenum Q176_PCEM33441 EPA 210222 SxF 4_FS M.raw 23.feb.21 FS Sandra yes MD009 SxF5_M_FS Sexy-fungi extract line 5 Penicillium chrysogenum Q176_PCEM33451 EPA 210222 SxF 5_FS M.raw 23.feb.21 FS Sandra yes MD010 SxF6_M_FS Sexy-fungi extract line 6 Penicillium chrysogenum Q176_PCEM33461 EPA 210222 SxF 6_FS M.raw 23.feb.21 FS Sandra yes MD011 WT_M_FS_2 wild type fungi extract Penicillium chrysogenum WT EPA 210906 WT M_FS.raw 6.sep.21 FS Sandra yes MD012 SxF3_M_FS_2 Sexy-fungi extract line 3 Penicillium chrysogenum Q176_PCEM33431 EPA 210906 SxF3 M_FS.raw 6.sep.21 FS Sandra yes MD013 SxF4_M_FS_2 Sexy-fungi extract line 4 Penicillium chrysogenum Q176_PCEM33441 EPA 210908 SxF4 M_FS.raw 8.sep.21 FS Sandra yes MD014 SxF5_M_FS_2 Sexy-fungi extract line 5 Penicillium chrysogenum Q176_PCEM33451 EPA 210909 SxF5 M FS.raw 9.sep.21 FS Sandra yes MD015 WT_M_2 wild type fungi extract Penicillium chrysogenum WT EPA 210908 WT M.raw 8.sep.21 SRM Sandra yes MD016 SxF3_M_2 Sexy-fungi extract line 3 Penicillium chrysogenum Q176_PCEM33431 EPA 210908 SxF3 M.raw 8.sep.21 SRM Sandra yes MD017 SxF4_M_2 Sexy-fungi extract line 4 Penicillium chrysogenum Q176_PCEM33441 EPA 210908 SxF4 M.raw 8.sep.21 SRM Sandra yes MD018 SxF5_M_2 Sexy-fungi extract line 5 Penicillium chrysogenum Q176_PCEM33451 EPA 210909 SxF5 M.raw 9.sep.21 SRM Sandra yes C12_1_2 C12_1.2 Transformant for production of Z-11-Hexadecen-1-ol pheromone (production not achieved) Penicillium digitatum PDEC12512 CSIC PHI26 strain transformed with FB125 to express AtrD11, HarFar and hygromycin resistance C12_2_1 C12_2.1 Transformant for production of Z-11-Hexadecen-1-ol pheromone (production not achieved) Penicillium digitatum PDEC12521 CSIC PHI26 strain transformed with FB125 to express AtrD11, HarFar and hygromycin resistance C12_5_1 C12_5.1 Transformant for production of Z-11-Hexadecen-1-ol pheromone (production not achieved) Penicillium digitatum PDEC12551 CSIC PHI26 strain transformed with FB125 to express AtrD11, HarFar and hygromycin resistance C123_1_1 C123_1.1 Transformant for production of Z-11-Hexadecen-1-ol and Z-11-hexadecenyl acetate pheromones (production not achieved) Penicillium digitatum PDEC126411 CSIC PHI26 strain transformed with FB125 to express AtrD11, HarFar, EaDact and hygromycin resistance [wrong assembly, EaDact not present] C123_4_2 C123_4.2 Transformant for production of Z-11-Hexadecen-1-ol and Z-11-hexadecenyl acetate pheromones (production not achieved) Penicillium digitatum PDEC12642 CSIC PHI26 strain transformed with FB125 to express AtrD11, HarFar, EaDact and hygromycin resistance [wrong assembly, EaDact not present] SxF3 SxF3 Transformant for production of moth pheromones Z-11-Hexadecen-1-ol and Z-11-Hexadecenyl acetate (production not achieved) Penicillium chrysogenum PCEM33431 CSIC Q176?paf strain transformed with FB334 to express Atr?11, HarFAR, ATF1 and geneticin resistance SxF4 SxF4 Transformant for production of moth pheromones Z-11-Hexadecen-1-ol and Z-11-Hexadecenyl acetate (production not achieved) Penicillium chrysogenum PCEM33441 CSIC Q176?paf strain transformed with FB334 to express Atr?11, HarFAR, ATF1 and geneticin resistance SxF5 SxF5 Transformant for production of moth pheromones Z-11-Hexadecen-1-ol and Z-11-Hexadecenyl acetate (production not achieved) Penicillium chrysogenum PCEM33451 CSIC Q176?paf strain transformed with FB334 to express Atr?11, HarFAR, ATF1 and geneticin resistance SxF6 SxF6 Transformant for production of moth pheromones Z-11-Hexadecen-1-ol and Z-11-Hexadecenyl acetate (production not achieved) Penicillium chrysogenum PCEM33461 CSIC Q176?paf strain transformed with FB334 to express Atr?11, HarFAR, ATF1 and geneticin resistance
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Created: 12th Apr 2022 at 16:00
Last updated: 7th Nov 2024 at 10:04
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Version 1 (earliest) Created 12th Apr 2022 at 16:00 by Marko Petek
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https://orcid.org/0000-0003-3644-7827PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.
Synthetic biology data management spreadsheet: https://fairdomhub.org/data_files/6036
Programme: NIBSys
Public web page: http://susphire.info/susphireproject/
Start date: 1st Apr 2018
End date: 30th Sep 2021
Short Name: T23_SxF Title: Moth pheromone production in filamentous fungi SxF Description: Demonstration of moth pheromone production in filamentous fungi by solvent extractions of liquid cultures and mycelial biomass and GC-MS/MS analysis. Constitutive and inducible expression of moth pheromones in filamentous fungi Penicillium species will be developed using a dCas9-based copper inducible system. An initial proof of concept will also be performed in P. digitatum using constitutive promoters. ...
Submitter: Marko Petek
Studies: Investigation files, _S_P1_ConstExp_Pdigitatum, _S_P5_SxFliquids, _S_P5_SxFmycelia
Assays: _A_FS-GC-MS, _A_FSM-GC-MS, _A_SRM-GC-MS, _A_SRMM-GC-MS, _A_qRTPCRMothPheromonesEnzymes-Q-RT-PCR, _I_T23_SxF-files, _S_P1_ConstExp_Pdigitatum-files, _S_P5_SxFliquids-files, _S_P5_SxFmycelia-files
Investigation files _I_T23_SxF
Files connected to investigation: _I_T23_SxF
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T23_SxF
Study: Investigation files
