SOPs

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Trypanothione synthetase enzymatic assay

SilicoTryp

This is a protocol for determining the activity of the T. brucei Trypanothione synthetase under in vivo-like conditions.

Creators: Alejandro Leroux, Luise Krauth-Siegel

Submitter: Alejandro Leroux

Investigations: Kinetic understanding of the T. brucei trypanot...

Studies: Kinetic characterization of trypanothione-depen...

Assays: Trypanothione synthetase ATP consumption steady..., Trypanothione synthetase Gsp and T(SH)2 product...

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Created: 23rd Nov 2012 at 15:57, Last updated: 27th Sep 2013 at 07:58

In vivo-like buffer for enzymatic measurements

SilicoTryp

This method describes the preparation of the in vivo-like buffer for the measurement of bloodstream T. brucei recombinant enzymes under pseudo-physiological conditions.