Technology type 'Initial Rate Experiment'

Enzymatic Activity Measurements
Current technology type

Initial Rate Experiment
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Related assays

7 Assays visible to you, out of a total of 9

Determination of intracellular redox state by means of NAD/NADH ratio

SUMO

This assay describes the determination of concentrations and ratio of metabolites of adenine nucleotides (NAD and NADH). These metabolites have been extracted from Escherichia coli MG1655 and isgenic mutant strains.

Submitter: Stefan Stagge

Assay type: Metabolomics

Technology type: Initial Rate Experiment

Investigation: Steady state studies for different oxygen avail...

Study: The Escherichia coli steady state response to o...

Organisms: Escherichia coli : K-12/MG1655 (wild-type / wild-type)

SOPs: 1 hidden item

Data files: 2 hidden items

Snapshots: No snapshots

Created: 11th May 2010 at 14:38, Last updated: 8th Nov 2017 at 14:21

Characterization of enzymes involved in butanediol formation

SysMO-LAB

Enzymes involved in butanediol formation from pyruvate in L. Lactis and E. faecalis were characterized with respect to their Km's for their substrates and their Vmaxes

Submitter: Martijn Bekker

Assay type: Experimental Assay Type

Technology type: Initial Rate Experiment

Investigation: The Attic

Study: Pre-liminary data from Martijn Bekker

Organisms: No organisms

SOPs: No SOPs

Data files: No Data files

Snapshots: No snapshots

Created: 11th Jun 2010 at 08:52, Last updated: 8th Nov 2017 at 14:21

PGK

SulfoSys

Kinetic characterisation of phosphoglycerate kinase

Submitter: Jacky Snoep

Assay type: Enzymatic Assay

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

SOPs: Cloning and heterologous expression in E. coli, Preparation of cell extracts of recombinant E.coli, Protein purification from E. coli extracts

Data files: PGK Kinetic Data

Snapshots: No snapshots

Created: 1st Aug 2013 at 09:14, Last updated: 8th Nov 2017 at 14:21

GAPDH

SulfoSys

Kinetic characterisation of glyceraldehyde 3-phosphate dehydrogenase

Submitter: Jacky Snoep

Assay type: Enzymatic Assay

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

SOPs: Cloning and heterologous expression in E. coli, Preparation of cell extracts of recombinant E.coli, Protein purification from E. coli extracts

Data files: GAPDH Kinetic Data

Snapshots: No snapshots

Created: 1st Aug 2013 at 11:37, Last updated: 8th Nov 2017 at 14:21

TPI

SulfoSys

Kinetic characterisation of triose phosphate isomerase

Submitter: Jacky Snoep

Assay type: Enzymatic Assay

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

SOPs: Cloning and heterologous expression in E. coli, Preparation of cell extracts of recombinant E.coli, Protein purification from E. coli extracts

Data files: TPI Kinetic Data

Snapshots: No snapshots

Created: 1st Aug 2013 at 14:02, Last updated: 8th Nov 2017 at 14:21

FBPAase

SulfoSys

Kinetic characterisation of fructose 1,6-bisphosphate aldolase phosphatase

Submitter: Jacky Snoep

Assay type: Enzymatic Assay

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

SOPs: Cloning and heterologous expression in E. coli, Preparation of cell extracts of recombinant E.coli, Protein purification from E. coli extracts

Data files: FBPAase Kinetic data

Snapshots: No snapshots

Created: 1st Aug 2013 at 14:05, Last updated: 8th Nov 2017 at 14:21

PPK kinetics data files and model files

Computational pathway design for biotechnological applications

PPK kinetic data for initial rate experiments and for enzyme incubations are linked and available in an Excel file. In addition a model file is available as Mathematica notebook for the parameterization of a rate equation for the enzyme, and for simulation of the enzyme incubations.

Submitter: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Initial Rate Experiment

Investigation: Polyphosphate kinases in Sulfolobus acidocaldarius

Study: PPK kinetics and incubations

Organisms: No organisms

SOPs: No SOPs

Data files: Experimental data file PPK kinetics, model file PPK incubations, model file for PPK kinetics

Snapshots: No snapshots

Created: 30th Jan 2026 at 13:52, Last updated: 30th Jan 2026 at 14:07