Assays
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Enzymes involved in butanediol formation from pyruvate in L. Lactis and E. faecalis were characterized with respect to their Km's for their substrates and their Vmaxes
Submitter: Martijn Bekker
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: The Attic
In this experiment we glucose-pulsed an E. faecalis cultures re-suspended in 100 mM MES buffer at pH 6.5. Samples were taken in time to study intra- and extracellular metabolites. These data are used to construct a kinetic model of the catqabolism of E. faecalis
Submitter: Martijn Bekker
Assay type: Metabolomics
Technology type: Progressive Curve Experiment
Investigation: The Attic
In this experiment we glucose-pulsed an L. lactiss cultures re-suspended in 100 mM MES buffer at pH 6.5. Samples were taken in time to study intra- and extracellular metabolites. These data are used to construct a kinetic model of the catabolism of E. L. lactis
Submitter: Martijn Bekker
Assay type: Metabolomics
Technology type: Enzymatic Activity Measurements
Investigation: The Attic