Kinetic characterisation

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Cyanobacteria are the only prokaryotes performing oxygenic photosynthesis. Theyhad and have tremendous influence on the biogeochemical cycles on Earth. Recently,cyanobacteria are increasingly investigated as cell factories for a sustainableeconomy. Despite their global, environmental and rising economic importance, ourknowledge on the regulation of their primary metabolism is fragmented.Cyanobacteria switch between photoautotrophic and heterotrophic modes ofmetabolism during day/night cycles or ...

These investigations reveal that Synechocystis has neither an ED pathway nor a glucose dehydrogenase/glucokinase (GHD/GK) bypass. It rather contains a promiscuous aldolase EDA that prefers KDPG as substrate but also decarboxylates oxaloacetate (OAA) and cleaves 2-keto-4-hydroxyglutarate (KHG). In addition, the synthesis of KDPG from pyruvate and GAP is catalyzed with very low efficiency.

Experimental data for the initial rate kinetics of EDA for KDPG, OAA, and KHG.

Mathematica notebook for MM model fit to the initial rate kinetics of EDA

Mathematica notebook for kinetic analysis of initial rate kinetics of the EDA