Snapshot 1
DownloadDOI: 10.15490/fairdomhub.1.investigation.775.1
Zenodo URL: None
Created at: 16th Jul 2026 at 19:00
The cyanobacterium Synechocystis has a promiscuous “ED”-aldolase but no functional Entner-Doudoroff pathway
These investigations reveal that Synechocystis has neither an ED pathway nor a glucose dehydrogenase/glucokinase (GHD/GK) bypass. It rather contains a promiscuous aldolase EDA that prefers KDPG as substrate but also decarboxylates oxaloacetate (OAA) and cleaves 2-keto-4-hydroxyglutarate (KHG). In addition, the synthesis of KDPG from pyruvate and GAP is catalyzed with very low efficiency.
Kinetic characterisation
Experimental data for initial rate kinetics of EDA (Sll0107) from Synechocystis for KDPG, OAA, and KHG, with a mathematical model based on MM kinetics to estimate the Vm and Km values.
Experimental data EDA kinetics
Experimental data for the initial rate kinetics of EDA for KDPG, OAA, and KHG.
Mathematical model for EDA kinetic analysis
Mathematica notebook for MM model fit to the initial rate kinetics of EDA
Mathematical model for EDA analysis
Mathematica notebook for kinetic analysis of initial rate kinetics of the EDA
- eda-kinetic-analysis.nb
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Created: 16th Jul 2026 at 19:00
Last updated: 16th Jul 2026 at 19:00
