EDA characterization for modelling

Version 1

The Deutsche Forschungsgemeinschaft (DFG) is the self-governing organisation for science and research in Germany. It serves all branches of science and the humanities. In organisational terms, the DFG is an association under private law. Its membership consists of German research universities, non-university research institutions, scientific associations and the Academies of Science and the Humanities.

Cyanobacteria are the only prokaryotes performing oxygenic photosynthesis. Theyhad and have tremendous influence on the biogeochemical cycles on Earth. Recently,cyanobacteria are increasingly investigated as cell factories for a sustainableeconomy. Despite their global, environmental and rising economic importance, ourknowledge on the regulation of their primary metabolism is fragmented.Cyanobacteria switch between photoautotrophic and heterotrophic modes ofmetabolism during day/night cycles or ...

These investigations reveal that Synechocystis has neither an ED pathway nor a glucose dehydrogenase/glucokinase (GHD/GK) bypass. It rather contains a promiscuous aldolase EDA that prefers KDPG as substrate but also decarboxylates oxaloacetate (OAA) and cleaves 2-keto-4-hydroxyglutarate (KHG). In addition, the synthesis of KDPG from pyruvate and GAP is catalyzed with very low efficiency.

Experimental data for initial rate kinetics of EDA (Sll0107) from Synechocystis for KDPG, OAA, and KHG, with a mathematical model based on MM kinetics to estimate the Vm and Km values.

Experimental data for the initial rate kinetics of EDA for KDPG, OAA, and KHG.

Mathematica notebook for MM model fit to the initial rate kinetics of EDA