SOPs

6 SOPs visible to you, out of a total of 6

Quenching and Extraction of intracellular metabolites

SUMO

This method describes how one can quench metabolism of Escherichia coli and extract metabolites from many kinds of metabolite classes like: nucleotides, sugar-phosphates, organic acids ....

Creator: Stefan Stagge

Submitter: Stefan Stagge

Investigations: Steady state studies for different oxygen avail...

Studies: Determination of the impact of specific enzyme ...

Assays: Determination of intracellular metabolite conce...

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Created: 11th May 2010 at 14:13, Last updated: 14th Jan 2013 at 17:02

Measurement of extracellular metabolites

SUMO

No description specified

Creator: Sonja Steinsiek

Submitter: The JERM Harvester

Investigations: Analysis of Escherichia coli with linear electr..., Analysis of the glucose transport in Escherichi..., Steady state studies for different oxygen avail...

Studies: Analysis of Escherichia coli strains with linea..., Characterization of mutant strains with defects..., Determination of the impact of specific enzyme ...

Assays: Aerobic and anaerobic characterization of MG165..., Analysis of by-product formation rates in MG1655, Characterization of E. coli MG1655 and ∆sdhC an..., Determination of by-product formation and gluco...

Created: 16th Mar 2010 at 17:13

Evans Medium

SUMO

No description specified

Creator: Katja Bettenbrock

Submitter: The JERM Harvester

Investigations: Analysis of Escherichia coli with linear electr..., Dynamical studies for different oxygen availabi..., Steady state studies for different oxygen avail...

Studies: Analysis of Escherichia coli strains with linea..., Determination of the impact of specific enzyme ..., The Escherichia coli dynamical response to oxyg..., The Escherichia coli steady state response to o...

Assays: Analysis of by-product formation rates in MG1655, Analysis of gene expression rates at different ..., ArcA phosphorylation at different aerobiosis le..., Characterization of E. coli MG1655 and ∆sdhC an..., FNR activity at different aerobiosis levels (st..., Gene expression analysis of mutants with linear..., Physiological measurements from Sheffield chemo..., Transcriptional profiling of E. coli during ana...

Created: 16th Mar 2010 at 17:13

DCW measurement

SUMO

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Steady state studies for different oxygen avail...

Studies: Determination of the impact of specific enzyme ..., The Escherichia coli steady state response to o...

Assays: Analysis of by-product formation rates in MG1655, Determination of intracellular metabolite conce..., Determination of intracellular redox state by m..., FNR activity at different aerobiosis levels (st..., Physiological measurements from Sheffield chemo...

Created: 16th Mar 2010 at 17:12

SUMO mRNA isolation Epicentre

SUMO

This file describes how to isolate mRNA from E. coli using the kit from Epicentre, for gene expression analysis via RT-PCR

Creator: Sonja Steinsiek

Submitter: Sonja Steinsiek

Investigations: Analysis of Escherichia coli with linear electr..., Analysis of the glucose transport in Escherichi..., Steady state studies for different oxygen avail...

Studies: Analysis of Escherichia coli strains with linea..., Characterization of mutant strains with defects..., Determination of the impact of specific enzyme ...

Assays: Aerobic and anaerobic characterization of MG165..., Analysis of gene expression rates at different ..., Characterization of MG1655 and mutant strains u..., Gene expression analysis of mutants with linear...

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Created: 26th Oct 2009 at 14:48

SUMO chemostat conditions

SUMO

This document describes the chemostat conditions to run comparable experiments in different bioreactors in different labs.

Creator: Sonja Steinsiek

Submitter: Sonja Steinsiek

Investigations: Analysis of Escherichia coli with linear electr..., Analysis of the glucose transport in Escherichi..., Dynamical studies for different oxygen availabi..., Steady state studies for different oxygen avail...

Studies: Analysis of Escherichia coli strains with linea..., Characterization of mutant strains with defects..., Determination of the impact of specific enzyme ..., The Escherichia coli dynamical response to oxyg..., The Escherichia coli steady state response to o...

Assays: Analysis of by-product formation rates in MG1655, Analysis of gene expression rates at different ..., ArcA phosphorylation at different aerobiosis le..., Characterization of MG1655 and mutant strains u..., Determination of by-product formation and gluco..., Determination of intracellular metabolite conce..., Determination of intracellular redox state by m..., Gene expression analysis of mutants with linear..., Physiological measurements from Sheffield chemo..., Transcriptional profiling of E. coli during ana...

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Created: 26th Oct 2009 at 14:48

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