Assays

4 Assays visible to you, out of a total of 4

Gene expression analysis of mutants with linear electron transport chain at different aerobiosis levels

SUMO

Mutant strains with linear electron transport chain were grown in chemostat cultures at different defined aerobiosis levels. Expression of selected genes was determined by Real Time RT-PCR

Submitter: Katja Bettenbrock

Assay type: Experimental Assay Type

Technology type: qRT-PCR

Investigation: Analysis of Escherichia coli with linear electr...

Study: Analysis of Escherichia coli strains with linea...

Organisms: No organisms

SOPs: Evans Medium, SUMO chemostat conditions, SUMO mRNA isolation Epicentre

Data files: ETCmutants_mRNA, Gene expression levels in mutants with linear r...

Snapshots: No snapshots

Created: 19th May 2014 at 08:56, Last updated: 8th Nov 2017 at 14:21

Characterization of MG1655 and mutant strains under conditions of glucose excess and limitation.

SUMO

Mutant strains which lack one or more of the glucose transport systems were analyzed in aerobic chemostat cultures and compared to batch cultures.

Submitter: Sonja Steinsiek

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Analysis of the glucose transport in Escherichi...

Study: Characterization of mutant strains with defects...

Organisms: No organisms

SOPs: SUMO chemostat conditions, SUMO mRNA isolation Epicentre

Data files: No Data files

Snapshots: No snapshots

Created: 17th Jan 2013 at 12:47, Last updated: 8th Nov 2017 at 14:21

Aerobic and anaerobic characterization of MG1655 and mutant strains with regard to transcription profiles.

SUMO

MG1655 and mutant strains with defects in glucose transport systems were analyzed in aerobic and anaerobic batch cultures.

Submitter: Sonja Steinsiek

Assay type: Gene Expression Profiling

Technology type: Technology Type

Investigation: Analysis of the glucose transport in Escherichi...

Study: Characterization of mutant strains with defects...

Organisms: No organisms

SOPs: SUMO mRNA isolation Epicentre and 1 hidden item

Data files: Transcription of glucose uptake transport syste..., Transcription of glucose uptake transport syste...

Snapshots: No snapshots

Created: 17th Jan 2013 at 13:06, Last updated: 8th Nov 2017 at 14:21

Analysis of gene expression rates at different aerobiosis levels via RT-PCR

SUMO

This assay describes how to analyze gene expression rates via RT-PCR.

Submitter: Sonja Steinsiek

Assay type: Gene Expression Profiling

Technology type: qRT-PCR

Investigation: Steady state studies for different oxygen avail...

Study: Determination of the impact of specific enzyme ...

Organisms: Escherichia coli

SOPs: Evans Medium, SUMO chemostat conditions, SUMO mRNA isolation Epicentre

Data files: Gene expression rates at different aerobiosis l... and 3 hidden items

Snapshots: No snapshots

Created: 21st Oct 2009 at 11:50, Last updated: 8th Nov 2017 at 14:21

Assays

Filters ×

Filters