Assays

What is an Assay?

9 Assays visible to you, out of a total of 9

Binding of NADP+ to Gre2p in PBS Buffer (ITC-BIND)

Towards Reproducible Enzyme Modeling

ITC binding (BIND) experiments to determine the binding parameters of NADP+ to Gre2p in 1x PBS Buffer.

Submitter: Gudrun Gygli

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Workflow for characterization of enzymes under ...

Study: ITC binding experiments

Organisms: No organisms

SOPs: SOP for ITC-BIND experiments, SOP for usage of the ITC

Data files: ITC-BIND experiment for NADP+ binding to Gre2p ..., ITC-BIND experiment for NADP+ binding to Gre2p ...

Snapshots: No snapshots

Created: 21st Apr 2021 at 12:12, Last updated: 5th May 2021 at 12:02

Binding of NADP+ to Gre2p in HEPES Buffer (ITC-BIND)

Towards Reproducible Enzyme Modeling

ITC binding (BIND) experiments to determine the binding parameters of NADP+ to Gre2p in 100 mM HEPES Buffer.

Submitter: Gudrun Gygli

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Workflow for characterization of enzymes under ...

Study: ITC binding experiments

Organisms: No organisms

SOPs: SOP for ITC-BIND experiments, SOP for usage of the ITC

Data files: ITC-BIND experiment for NADP+ binding to Gre2p ..., ITC-BIND experiment for NADP+ binding to Gre2p ...

Snapshots: No snapshots

Created: 21st Apr 2021 at 12:12, Last updated: 5th May 2021 at 12:02

Binding of NDK to Gre2p (ITC-BIND)

Towards Reproducible Enzyme Modeling

ITC binding (BIND) experiments to determine the binding parameters of NDK (nitrononane-2,8-dione) to Gre2p in 100 mM KPi Buffer. Experiments failed due to very weak binding and poor solubility of NDK in buffer.

Submitter: Gudrun Gygli

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Workflow for characterization of enzymes under ...

Study: ITC binding experiments

Organisms: No organisms

SOPs: SOP for ITC-BIND experiments, SOP for usage of the ITC

Data files: ITC-BIND experiment for NDK binding to Gre2p (...., ITC-BIND experiment for NDK binding to Gre2p (...., ITC-BIND experiment for NDK binding to Gre2p (...., ITC-BIND experiment for NDK binding to Gre2p (....

Snapshots: No snapshots

Created: 21st Apr 2021 at 12:14, Last updated: 5th May 2021 at 12:02

Binding of HK to Gre2p (ITC-BIND)

Towards Reproducible Enzyme Modeling

ITC binding (BIND) experiments to determine the binding parameters of HK ((5S,8S)-anti hydroxyketone) to Gre2p in 100 mM KPi Buffer. Experiments failed due to very weak binding and poor solubility of HK in buffer.

Submitter: Gudrun Gygli

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Workflow for characterization of enzymes under ...

Study: ITC binding experiments

Organisms: No organisms

SOPs: SOP for ITC-BIND experiments, SOP for usage of the ITC

Data files: ITC-BIND experiment for HK binding to Gre2p (.apj), ITC-BIND experiment for HK binding to Gre2p (.apj), ITC-BIND experiment for HK binding to Gre2p (.csv), ITC-BIND experiment for HK binding to Gre2p (.csv)

Snapshots: No snapshots

Created: 21st Apr 2021 at 12:15, Last updated: 5th May 2021 at 12:02

Binding of NADPH to Gre2p in Tween-KPi Buffer (ITC-BIND)

Towards Reproducible Enzyme Modeling