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1 Assay visible to you, out of a total of 1

Trypanothione synthetase Gsp and T(SH)2 production measured by HPLC

SilicoTryp

TbTryS activity was measured at 37°C in the in vivo-like buffer. All substrate stock solutions were prepared in the in vivo-like buffer and the pH was adjusted to 7.0. The assays were performed in a final volume of 2 ml and contained 0.2 mM NADH, 1 mM phosphoenolpyruvate, 4 units pyruvate kinase, 4 units L-lactate dehydrogenase, 0.17 µM TbTryS, 2.1 mM ATP and varying amounts of glutathione, and spermidine.

Submitter: Alejandro Leroux

Assay type: Discontinuous Enzymatic

Technology type: HPLC

Investigation: Kinetic understanding of the T. brucei trypanot...

Study: Kinetic characterization of trypanothione-depen...

Organisms: No organisms

SOPs: Derivatization of Gsp and T(SH)2 produced in th..., In vivo-like buffer for enzymatic measurements, Trypanothione synthetase enzymatic assay

Data files: Activity of TbTryS measured by HPLC

Snapshots: No snapshots

Created: 6th Aug 2014 at 13:27, Last updated: 8th Nov 2017 at 14:21

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