Assays
What is an Assay?Filters
Cell sorting is a flow cytometry-based assay used to physically separate heterogeneous cell populations into distinct subpopulations based on specific cell surface or intracellular markers, enabling downstream functional, molecular, or phenotypic analyses. Cells are labeled with fluorescently conjugated antibodies or dyes, interrogated by laser excitation, and individually sorted into collection vessels using electrostatic deflection or microfluidic mechanisms according to predefined gating ...
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Flow cytometry is a laser-based, high-throughput single-cell analysis technique used to simultaneously measure multiple physical and fluorescent characteristics of cells or particles in suspension. This assay quantifies cell populations based on parameters such as cell size (forward scatter), granularity (side scatter), and fluorescence intensity of labeled surface or intracellular markers, enabling immunophenotyping, cell cycle analysis, viability assessment, and functional studies. Samples are ...
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Flow cytometry is employed to quantitatively measure cell surface and/or intracellular markers, enabling characterization of cell populations based on fluorescence intensity and light scatter properties. This assay utilizes a flow cytometer to analyze individual cells in suspension, providing multi-parametric data on cell phenotype, viability, activation state, or other biological attributes as defined by the experimental panel. Samples are processed, stained with fluorescently labeled antibodies ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB