Assays
What is an Assay?Filters
Cell sorting is a flow cytometry-based assay used to physically separate heterogeneous cell populations into distinct subpopulations based on specific cell surface or intracellular markers, enabling downstream functional, molecular, or phenotypic analyses. Cells are labeled with fluorescently conjugated antibodies or dyes, interrogated by laser excitation, and individually sorted into collection vessels using electrostatic deflection or microfluidic mechanisms according to predefined gating ...
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Flow cytometry is a laser-based, high-throughput single-cell analysis technique used to simultaneously measure multiple physical and fluorescent characteristics of cells or particles in suspension. This assay quantifies cell populations based on parameters such as cell size (forward scatter), granularity (side scatter), and fluorescence intensity of labeled surface or intracellular markers, enabling immunophenotyping, cell cycle analysis, viability assessment, and functional studies. Samples are ...
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay captures and standardizes the metadata associated with cell extraction procedures, documenting key experimental parameters such as cell type, passage number, extraction method, reagents used, and processing conditions applied during the preparation of cellular material for downstream analyses. It serves as a critical data governance step to ensure traceability, reproducibility, and quality control across experiments by recording provenance information for extracted cell samples. The ...
Submitter: Dikshant Pradhan
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Short read sequencing is a high-throughput next-generation sequencing (NGS) approach used to determine the nucleotide sequence of DNA or RNA fragments, enabling detection of genetic variants, gene expression profiles, or genomic features depending on the library preparation strategy employed. This assay involves fragmentation of input nucleic acids, adapter ligation, and amplification prior to sequencing on short-read platforms (such as Illumina), generating millions of short sequence reads ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
This assay evaluates the ability of bacterial populations to survive under defined restrictive conditions, quantifying survivability metrics through linked experimental data to assess bacterial viability and growth inhibition. The methodology incorporates controlled exposure of bacterial cultures to restrictive agents or environmental stressors, with outcomes measured via standardized enumeration or optical density-based approaches to determine survival rates relative to untreated controls. Results ...
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB
Antibody Dependent Functional Profiling* and Analysis Represents:
ADMP: Macrophage Phagocytosis
ADNP: Neutrophil Phagocytosis
ADDCP: Dendritic Cell Phagocytosis
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: IMPAcTB