Assays

A mouse challenge assay introduces a defined treatment, infection, or condition to experimental mice. It assesses physiological, immunological, or pathological responses to controlled exposures. Input: Experimental mouse model Output: Treated or exposed mouse sample for downstream analysis.

Cell sorting is a flow cytometry-based assay used to physically separate heterogeneous cell populations into distinct subpopulations based on specific cell surface or intracellular markers, enabling downstream functional, molecular, or phenotypic analyses. Cells are labeled with fluorescently conjugated antibodies or dyes, interrogated by laser excitation, and individually sorted into collection vessels using electrostatic deflection or microfluidic mechanisms according to predefined gating ...

Flow cytometry is a laser-based, high-throughput single-cell analysis technique used to simultaneously measure multiple physical and fluorescent characteristics of cells or particles in suspension. This assay quantifies cell populations based on parameters such as cell size (forward scatter), granularity (side scatter), and fluorescence intensity of labeled surface or intracellular markers, enabling immunophenotyping, cell cycle analysis, viability assessment, and functional studies. Samples are ...

Western blot is a widely used immunodetection technique that separates proteins by molecular weight via gel electrophoresis, transfers them onto a membrane (typically nitrocellulose or PVDF), and detects specific target proteins using primary and secondary antibodies conjugated to reporter molecules such as horseradish peroxidase or fluorescent dyes. Sample preparation typically involves cell or tissue lysis, protein quantification, and denaturation in SDS-containing loading buffer prior to ...

This assay evaluates the ability of bacterial populations to survive under defined restrictive conditions, quantifying survivability metrics through linked experimental data to assess bacterial viability and growth inhibition. The methodology incorporates controlled exposure of bacterial cultures to restrictive agents or environmental stressors, with outcomes measured via standardized enumeration or optical density-based approaches to determine survival rates relative to untreated controls. Results ...

An antibody dependent complement deposition assay measures complement activation following antibody binding. The process detects deposition of complement proteins as an indicator of antibody-mediated immune function. Input: Antibody-treated target cells or particles Output: Complement deposition data file or fluorescence readout.

Antibody Dependent Functional Profiling* and Analysis Represents:

ADMP: Macrophage Phagocytosis

ADNP: Neutrophil Phagocytosis

ADDCP: Dendritic Cell Phagocytosis