Assays

5 Assays visible to you, out of a total of 5

Aerobic and anaerobic characterization of MG1655 and mutant strains with regard to growth, by-product formation and cAMP concentrations.

SUMO

Glucose transporter mutants were analyzed under aerobic and aerobic conditions in batch cultures with glucose as substrate. Acetate formation rates and glucose consumption rates were measured, as well as extracellular cAMP concentrations.

Submitter: Sonja Steinsiek

Assay type: Extracellular Metabolite Concentration

Technology type: Technology Type

Investigation: Analysis of the glucose transport in Escherichi...

Study: Characterization of mutant strains with defects...

Organisms: Escherichia coli : MG1655 (del LAM;del rph-1 / pyrimidine starvation )

SOPs: Measurement of extracellular metabolites and 1 hidden item

Data files: Growth, by-product formation and glucose consum..., Growth, yield and cAMP excretion under anaerobi...

Snapshots: No snapshots

Created: 17th Jan 2013 at 12:18, Last updated: 8th Nov 2017 at 14:21

Literature Data from Alexeeva et al., J. Bacteriol., 2000, 2002, 2003

SUMO

No description specified

Submitter: Michael Ederer

Assay type: Experimental Assay Type

Technology type: Technology Type

Investigation: Steady state studies for different oxygen avail...

Study: The Escherichia coli steady state response to o...

Organisms: No organisms

SOPs: No SOPs

Data files: Numeric Data from Alexeeva et al., J. Bacteriol...

Snapshots: No snapshots

Created: 25th Jan 2013 at 17:24, Last updated: 8th Nov 2017 at 14:21

ArcA phosphorylation at different aerobiosis levels (steady states)

SUMO

ArcA phosphorylation in chemostat cultures grown at different aerobiosis levels was quantitated by Phos-tag SDS-PAGE gel analysis and subsequent immunodetection of ArcA.

Submitter: Matthew Rolfe

Assay type: Experimental Assay Type

Technology type: SDS-PAGE

Investigation: Steady state studies for different oxygen avail...

Study: The Escherichia coli steady state response to o...

Organisms: Escherichia coli : MG1655 (del LAM;del rph-1 / pyrimidine starvation )

SOPs: Determination of protein phosphorylation levels..., Evans Medium, SUMO chemostat conditions

Data files: No Data files

Snapshots: No snapshots

Created: 30th Jan 2013 at 15:22, Last updated: 8th Nov 2017 at 14:21

Determination of intracellular redox state by means of ubiquinones (oxd/red-ratio)

SUMO

This assay describes the determination of concentrations and ratio of metabolites of ubiquinones (oxidised and reduced form). These metabolites have been extracted from Escherichia coli MG1655 and isgenic mutant strains.

Submitter: Stefan Stagge

Assay type: Metabolomics

Technology type: HPLC

Investigation: Steady state studies for different oxygen avail...

Study: The Escherichia coli steady state response to o...

Organisms: Escherichia coli : K-12/MG1655 (wild-type / wild-type)

SOPs: DCW measurement, Q/QH2 sampling, SUMO chemostat conditions

Data files: 091016 quinones complete data magdeburg, 091016 quinones complete data magdeburg

Snapshots: No snapshots

Created: 12th May 2010 at 14:49, Last updated: 8th Nov 2017 at 14:21