Assays

Files connected to study: _S_P5_SxF_lavandulol

Short Name: LA_myc-GC-MS Assay Class: WET Assay Type: GC-MS Title: Detection of lavandulyl acetate in SxF mycelium extracts Description: Detection of lavandulyl acetate in solvent extracts of SxF mycelia by GC-MS-MS pISA Assay creation date: 2021-12-23 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data: https://doi.org/10.5281/zenodo.6559159

Short Name: L_liq-GC-MS Assay Class: WET Assay Type: GC-MS Title: Detection of lavandulol in extracts of SxF liquid cultures Description: Detection of lavandulol in solvent extracts of SxF liquid cultures by GC-MS-MS pISA Assay creation date: 2021-12-23 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data: https://doi.org/10.5281/zenodo.6559450

Short Name: L_myc-GC-MS Assay Class: WET Assay Type: GC-MS Title: Dectection of lavandulol in SxF mycelium extracts Description: Detection of lavandulol in solvent extracts of SxF mycelia by GC-MS-MS pISA Assay creation date: 2021-12-23 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data: https://doi.org/10.5281/zenodo.6559481

Files connected to investigation: _I_T33_MonoterpenoidsFungi

Files connected to study: _S_P5_SxF_lav_ac

Short Name: LA_liq-GC-MS Assay Class: WET Assay Type: GC-MS Title: Detection of lavandulyl acetate in extracts of SxF liquid cultures Description: Detection of lavandulyl acetate in solvent extracts of SxF liquid cultures by GC-MS-MS pISA Assay creation date: 2021-12-23 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data: https://doi.org/10.5281/zenodo.6559101

Files connected to investigation: _I_T32_CandidateGeneExpressionTesting

Files connected to study: _S_TransientLPPSCPPS

Short Name: TransientCompLPPSCPPS-GCMS Assay Class: WET Assay Type: GCMS Title: Transient expression of LPPS and CPPS genes in Nicotiana benthamiana plants Description: The purpose of this assay is to check the transient expression of the LPPS and CPPS genes in Nicotiana benthamiana plants. Two different Agrobacterium strains were assayed and compared the levels of production between them. Volatile monoterpenoid levels were measured by GCMS of samples. pISA Assay creation date: 2021-12-03 pISA ...

Files connected to study: _S_P5_PhEmissionPlants

Short Name: emission_nontruncated-GC-MS Assay Class: WET Assay Type: GC-MS Title: Pheromone emission of non-truncated SxP plants Description: Quantification of the pheromone emitted by the non-truncated SxP versions that were producing both Z11C16OL and Z11C16AC pISA Assay creation date: 21-Nov-16 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: emission_truncated-GC-MS Assay Class: WET Assay Type: GC-MS Title: Pheromone emitted by truncated versions of SxP Description: Quantification of the pheromone emitted by truncated versions of SxP plants that were not producing Z11C16AC pISA Assay creation date: 21-Nov-16 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Files connected to study: _S_P5_PhContentPlants

Short Name: GC-MS-EAD-EAG Assay Class: WET Assay Type: EAG Title: EAG recordings coupled to GC-MS Description: EAG recordings of stimuli provided to Sesamia nonagrioides male antenna by the effluent of the GC-MS pISA Assay creation date: 21-Nov-16 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: PhCont_fr20-GC-MS Assay Class: WET Assay Type: GC-MS Title: Quantification of pheromone content in leaves frozen at -20 Description: Quantification of the pheromone content in SxP v1.2 plant leaves frozen at -20C that produce both Z11C16OL nad Z11C16AC pISA Assay creation date: 21-Nov-17 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: PhCont_fr80-GC-MS Assay Class: WET Assay Type: GC-MS Title: Quantification of pheromone content in leaves frozen at -80 Description: Quantifiction of pheromone content in SxP v1.2 plant leaves frozen at -80C that produce both Z11C16OL and Z11C16AC pISA Assay creation date: 21-Nov-17 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: PhCont_fresh-GC-MS Assay Class: WET Assay Type: GC-MS Title: Pheromone content in fresh leaves Description: Quantification of the pheromone content in fresh leaves of SxP v1.2 plants that produce both Z11C16OL and Z11C16AC pISA Assay creation date: 21-Nov-17 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Files connected to investigation: _I_T24_phero

Files connected to study: _S_P5_EAGresponse

Short Name: EAG_manual-EAG Assay Class: WET Assay Type: EAG Title: EAG recordings of manual stimuli Description: EAG recordings of stimulus manually provided to Sesamia nonagrioides male antenna pISA Assay creation date: 21-Nov-16 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Files connected to study: _S_P5_SxFliquids

Files connected to study: _S_P5_SxFmycelia

Short Name: FS-GC-MS Assay Class: WET Assay Type: GC-MS Title: Full Scan analysis of SxF liquid samples Description: Full Scan analysis of the solvent extracts of SxF liquid samples pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: SRM-GC-MS Assay Class: WET Assay Type: GC-MS Title: SRM analysis of SxF liquid samples Description: GC-MS analysis in SRM mode of the solvent extracts of SxF liquid samples pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: FSM-GC-MS Assay Class: WET Assay Type: GC-MS Title: Full Scan analysis of SxF mycelium extracts Description: GC-MS analysis in full scan mode of SxF mycelium solvent extracts pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Short Name: SRMM-GC-MS Assay Class: WET Assay Type: GC-MS Title: SRM analysis on SxF mycelium extracts Description: GC-MS analysis in SRM mode of SxF mycelium extracts pISA Assay creation date: 21-Sep-27 pISA Assay creator: Sandra Vacas Phenodata: None Featuredata: Data:

Files connected to investigation: _I_T23_SxF

Files connected to study: _S_P1_ConstExp_Pdigitatum

Short Name: qRTPCRMothPheromonesEnzymes-Q-RT-PCR Assay Class: WET Assay Type: Q-RT-PCR Title: qRT-PCR Analysis of enzymes AtrD11 and HarFar in Penicillium digitatum transformants Description: Two Penicillium digitatum transformants were selected that showed integration of the two genes AtrD11 and HarFar (PCR test) but not moth pheromone production (GC-MS, analysed in CEQA facilities). These two transformants were then assayed in their expression of the transgenes by qRT-PCR. pISA Assay creation ...