People

SysMO3

BaCell-SysMO2 SUMO1

University of Erlangen-Nuernberg2 University of Sheffield1

Germany2 United Kingdom1

Western blot analyses3

Western blot analyses3 Matlab31 Transcriptomics28 Biochemistry and protein analysis23 Molecular Biology22 Microbiology21 Computational and theoretical biology21 Fermentation21 ODE20 Molecular biology techniques (RNA/DNA/Protein)20 Microarray analysis17 Genetic modification15 Computational Systems Biology14 Mathematica13 Dynamic modelling13 Proteomics (2D-PAGE)11 Biochemistry10 Metabolomics10 Chromatography10 Proteomics9 SBML9 Copasi9 Stochastic models9 Genetics8 Bioinformatics8 R8 Fluorecence based reporter gene analyses/single cell analyses8 qRT-PCR8 Data Management7 Python7 linux7 Fluorescence and confocal microscopy7 Genomics6 Databases6 Java6 differential algebraic equations6 continuous cultivation6 molecular biological techniques (RNA/DNA techniques6 Mass spectrometry (LC-MS/MS)6 Genetic analysis5 Model organisms5 Partial differential equations5 Perl5 stimulus response experiments5 fluorescence spectroscopy5 quantitative western blot analysis5 Deterministic models5 HPLC5 Cell culture5 Spectroscopy and structural analysis4 Web services4 PCR4 Ruby on Rails4 Material balance based modeling4 Enzyme assay4 data modeling4 C programming4 MySQL4 Molecular biology techniques (RNA/DNA)4 time lapse microscopy4 2-D Gel Electrphoresis4 HTML4 Metabolic Engineering4 Bioconductor Packages in R4 microbiology techniques4 Parameter estimation4 Cell biology3 Single Cell analysis3 Workflows3 Chip-chip3 Taverna3 SQL3 evaluation of process dynamics3 Protein chemical methods (protein overproduction3 enzymatic analyses3 mutant strain generation3 quantitative western blot analyses3 XPPAUT3 Membrane protein biochemistry3 Flux balance analysis3 DNA technology RNA technology Protein analysis Fermentation Mutagenesis3 GC and LC analysis of metabolites3 Chemostat culture3 Mutant and Strain Construction3 Enzyme assays3 reporter gene analyses3 stochastic methods3 DNA3 analysis of functional genomics data3 interaction analysis techniques especially SPR measurements3 enzyme kinetics3 semantic web3 Javascript3 SEEK3 Algebraic equations2 Linear equations2 JWS Online2 rtPCR2 qtPCR2 Mass spectrometry2 Ruby2 Ontologies2 PHP2 DIVA2 quantitative Western analyses)2 qPCR2 Northern analyses)2 northern blot analysis2 including:- Dynamic modelling- Parameter estimation- Optimal experimental design- Dynamic optimization2 Biochemistry of substrate transport. molecular biological techniques (RNA/DNA techniques analyses of transport across cell membranes heterologous expression of transporters2 Chemical cross-linking2 Site-directed mutagenesis2 transcriptional analysis (Northern blots2 Optimal experimental design2 Proteomics (Glycoproteins)2 BioMart2 Comparative Genomics2 GC and LC analysis of metabolites | Analysis of signal molecules | Handling of anaerobic bacteria |2 SubtiWiki2 protein-DNA interactions; gel shifts; footprints; in vitro transcription protein ligan interactions2 absolute quantification2 Flow cytometry2 Immunofluorescence2 molecular biological techniques (RNA/DNA techniques)2 protein interaction studies2 In silico Metabolic Network Analysis2 fed-batch cultivation2 RNA / DNA Techniques2 bacterial chemostat culture2 spectroscopy2 site-directed and random mutagenesis2 Mutant and Strain Construction; RNA / DNA Techniques; RT-RealTime-PCR; 2D-Gels; Metabolite Analysis; Bioreactor Experiments; Flow Cyometry; Microscopy; Single Cell analysis2 Chemostats2 Proteins2 Gaschromatographic analysis2 gel-based and gel-free proteomics2 Statistical tools2 shotgun proteomics2 in vivo crosslinking2 PIPSA2 SYCAMORE2 fortran2 transcription analysis2 NMR2 Perl programming2 RNA interference2 protein purification2 phage techniques2 GC and LC/MS analysis of metabolites2 COBRA toolbox2 Cell and tissue culture1 Isolation purification and separation1 Pharmacology and toxicology1 Spring1 semanticSBML1 MIRIAM1 ProMOT1 J2EE1 pulsed - FRAP1 purification1 molecular biological techniques (RNA/DNA techniques analyses of transport across cell membranes heterologous expression of transporters1 cultivation1 Biochemistry of substrate transport. molecular biological techniques (RNA/DNA techniquesanalyses of transport across cell membranesheterologous expression of transporters1 FRAP1 including: - Dynamic modelling - Parameter estimation - Optimal experimental design - Dynamic optimization1 Fluorescence and confocal microscopy FRAP/FLIP1 FACS1 genomic array footprinting1 gene regulation1 The Membrane Enzymology group focuses on the molecular mechanisms of transport and signalling across biological membranes. In other words: How do molecules permeate biological membranes and how at the level of protein molecules are environmental signals s1 engineering principles and approaches from molecular biology and synthetic chemistry are applied. Confocal microscopy and various spectroscopic methods are used to analyze processes at the single cell or even single molecule level.1 PDE1 C programing1 quantitative RT-PCR); quantitative Western blot analysis; protein purification; reconstitution of membrane-integrated proteins; Biochemistry of substrate transport; Fluorecence based reporter gene analyses/single cell analyses1 surface plasmon resonance spectroscopy; site-directed spin labeling and EPR analysis;1 Dynamic optimization1 Systems Biology1 Time-lapse fluorescence microscopy Flow cytometry1 EnsEMBL1 XML1 Phylogenetics1 methods of nonlinear time series analysis1 programming in C1 site-directed spin labeling and EPR spectroscopy1 isothermal titration calorimetry1 Biophysics1 SPINE1 bacterial two-hybrid system1 bacterial chamostat culture1 time-resolved microarray analysis1 GeLC-MS/MS1 metabolic labeling1 AQUA1 Logical modelling1 trac (wiki for data management)1 Fluxomics1 Fluorescence microscopy1 transposon mutagenesis1 Time-lapse fluorescence microscopy1 FLAME - Flexible Large-scale Agent-based Modelling Framework.1 GFP expression1 live cell array1 X-ray crystallography electron microscopy Biochemistry and protein analysis1 Bioreactor experiments/Fermentation (batch1 Chemostat)1 2D-PAGE1 plasmon resonance spectroscopy1 phage transduction1 Molecular biology techniques (DNA/Protein)1 Chemostat culture GC and LC analysis of metabolites1 Multiscale and multiphysics modelling1 asymptotic methods1 network theory1 model reduction.1 RNA1 Gaschromatography1 Creation of Mutants1 stress experiments1 Groovy1 Protein1 Mutant construction1 2-D Gel Electrophoresis1 Chemostat cultures Molecular biology techniques (RNA/DNA) 2-D Gel electrophoresis Gaschromatography Protein overexpression/purification1 molecular biology techniques1 molecular biological1 microbiological and immunological techniques1 surface plasmon resonance spectroscopy1 reporter gene analysis1 X-ray crystallographyelectron microscopyBiochemistry and protein analysis1 Maple1 Clustering softwares1 Pathway Tools1 affinity copurification/precipitation1 model prediction testing1 reverse engineering1 computational platform development1 metabolic netwlrk visualization1 gPROMS1 SABIO-RK http://sabio.villa-bosch.de/1 Molecular Dynamics1 molecular modeling and simulation1 Protein and RNA structure prediction1 Homology Modelling1 Programming in perl and tcl1 Grails web development framework1 iTRAQ for quantitative proteomics1 madonna1 genetics of hyperthermophilic archaea1 phylogeny1 CUDA1 GPU1 Thermodynamics1 Machine Learning1 SABIO-RK database1 in vitro transcription1 DNA affinity chromatography1 EMSA1 RightField1 PySCeS1 RNAseq1 Bayesian inference1 Physiology1 Protege1 Bioreactor experiments1 web development1 AWS1 Research Objects1 SABIO-RK1 MS imaging1 FT-ICR-MS1 Field Flow Fractionation1 Docker1 Kinetic Modeling1 Data Modelling1 More...