Data files
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Badly chosen substrate concentrations for an intital rate experiment give data that makes it difficult to fit with a kinetic model and judge the quality of the fit.
Creator: Gudrun Gygli
Submitter: Gudrun Gygli
Well chosen substrate concentrations for an intital rate experiment give data that makes it easy to fit with a kinetic model and judge the quality of the fit and use it as a basis for the final gold-round experiment.
Creator: Gudrun Gygli
Submitter: Gudrun Gygli
Very well chosen substrate concentrations for an intital rate experiment give data that makes it very easy to fit with a kinetic model and judge the quality of the fit.
Creator: Gudrun Gygli
Submitter: Gudrun Gygli
An initial choice of 5 widely spaced substrates enables a first, rough estimate of Km to inform the design of first-round experiments.
Creator: Gudrun Gygli
Submitter: Gudrun Gygli
Raw data of the measurements obtained via DLS measurements. Can be opened with the Zetasizer software.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC recurrent single injection experiment for the reaction of NADPH and NDK with Gre2p in KPi, PBS and HEPES buffer (100, 1x, 100 mM, respectively, all at 25°C and pH 7.5). In this apj file, the data for the analysis of the first injection peak is stored. *.apj file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC recurrent single injection experiment for the reaction of NADPH and NDK with Gre2p in KPi, PBS and HEPES buffer (100, 1x, 100 mM, respectively, all at 25°C and pH 7.5). In this apj file, the data for the analysis of the second injection peak is stored. *.apj file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC recurrent single injection experiment for the reaction of NADPH and NDK with Gre2p in KPi, PBS and HEPES buffer (100, 1x, 100 mM, respectively, all at 25°C and pH 7.5). In this csv file, the data for the analysis of the both injection peaks is stored. *.csv file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC multiple injection experiment for the reaction of NADPH and NDK with Gre2p in 0.1%Tween-20-KPI buffer (100 mM, pH 7.5) at 25 °C. *.apj file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC multiple injection experiment for the reaction of NADPH and NDK with Gre2p in 0.1%Tween-20-KPI buffer (100 mM, pH 7.5) at 25 °C. *.csv file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
Raw data of the measurements obtained via DLS measurements. Can be opened with the Zetasizer software.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC multiple injection experiment for the reaction of NADPH and NDK with Gre2p in HEPES buffer (100 mM, pH 7.5) at 25 °C. *.apj file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC multiple injection experiment for the reaction of NADPH and NDK with Gre2p in HEPES buffer (100 mM, pH 7.5) at 25 °C. *.csv file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC multiple injection experiment for the reaction of NADPH and NDK with Gre2p in KPI buffer (100 mM, pH 7.5) at 25 °C. *.apj file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC multiple injection experiment for the reaction of NADPH and NDK with Gre2p in KPI buffer (100 mM, pH 7.5) at 25 °C. *.csv file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC multiple injection experiment for the reaction of NADPH and NDK with Gre2p in PBS buffer (1x, pH 7.5) at 25 °C. *.apj file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC multiple injection experiment for the reaction of NADPH and NDK with Gre2p in PBS buffer (1x, pH 7.5) at 25 °C. *.csv file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of (5S,8S)-anti hydroxyketone (HK) to Gre2p. Note that binding was unsuccessful. *.apj file, experiment titrating 1 mM HK.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of (5S,8S)-anti hydroxyketone (HK) to Gre2p. Note that binding was unsuccessful. *.apj file, experiment titrating 200 mM HK.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of (5S,8S)-anti hydroxyketone (HK) to Gre2p. Note that binding was unsuccessful. *.csvfile, experiment titrating 1 mM HK.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of (5S,8S)-anti hydroxyketone (HK) to Gre2p. Note that binding was unsuccessful. *.csv file, experiment titrating 200 mM HK.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of NADPH to Gre2p in 0.1% Tween-20 - KPi buffer (100 mM, pH 7.5) at 25 °C. *.apj file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of NADPH to Gre2p in 0.1% Tween-20 - KPi buffer (100 mM, pH 7.5) at 25 °C. *.csv file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of NADP+ to Gre2p in HEPES buffer (100 mM, pH 7.5) at 25 °C. *.apj file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of nitrononane-2,8-dione (NDK) to Gre2p. Note that binding was unsuccessful. *.apj file, experiment titrating 1 mM NDK.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of nitrononane-2,8-dione (NDK) to Gre2p. Note that binding was unsuccessful. *.apj file, experiment titrating 100 mM NDK.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of NADP+ to Gre2p in HEPES buffer (100 mM, pH 7.5) at 25 °C. *.csv file.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of nitrononane-2,8-dione (NDK) to Gre2p. Note that binding was unsuccessful. *.csv file, experiment titrating 1 mM NDK.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of nitrononane-2,8-dione (NDK) to Gre2p. Note that binding was unsuccessful. *.csv file, experiment titrating 100 mM NDK.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
ITC binding experiment for the binding of NADPH to Gre2p in HEPES buffer (100 mM, pH 7.5) at 25 °C. *.apj file
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli