Assays

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1 Assay visible to you, out of a total of 1

BaCell-SysMO

We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity.

There occurs an unexpected drop in the beta-Gal activity after sigB induction. This modelling effort aims to clarify the reasons.

Submitter: Ulf Liebal

Biological problem addressed: Gene Expression

Investigation: The transition from growing to non-growing Baci...

Study: Controlled sigmaB induction in shake flask

Organisms: Bacillus subtilis : 168 (wild-type / wild-type)

Models: BSA115 Post-transcriptional instability, BSA115-lacz-synth, BSA115-sigB-adaptation, BSA115-transcription inhibition model

SOPs: No SOPs

Data files: 20090915_BSA115-IPTG-assay, Model-representations-BSA115-Experiments and 1 hidden item

Snapshots: No snapshots

Created: 2nd Feb 2011 at 14:07, Last updated: 8th Nov 2017 at 14:21