SOPs
What is a SOP?Filters
The phosphorylation level of a particular protein can be determined using a procedure based upon western immunoblotting, with Phos-tag™ reagent present in the SDS-PAGE gel. The Phos-tag™ reagent, supplied in the form of Phos-tag™ acrylamide (Wako Pure Chemical Industries, AAL-107), causes proteins to be resolved both on the basis of size and phosphorylation state. This means that phosphorylated and de-phosphorylated forms of the same protein can be distinguished.
Creator: Matthew Rolfe
Submitter: Matthew Rolfe
This method describes how one can quench metabolism of Escherichia coli and extract metabolites from many kinds of metabolite classes like: nucleotides, sugar-phosphates, organic acids ....
Creator: Stefan Stagge
Submitter: Stefan Stagge
This protocol describes the transcriptional profiling of E. coli cultures using microarrays. The protocol utilises RNA isolated as described in another SOP (SUMO RNA isolation from E. coli) and with hybridisation to Ocimum Ocichip E. coli K-12 microarrays.
Creator: Matthew Rolfe
Submitter: Matthew Rolfe
This SOP describes the preparation of E. coli RNA
Creator: Matthew Rolfe
Submitter: Matthew Rolfe
Creator: Martijn Bekker
Submitter: The JERM Harvester
Creator: Sonja Steinsiek
Submitter: The JERM Harvester
Creator: Katja Bettenbrock
Submitter: The JERM Harvester
Creator: Martijn Bekker
Submitter: The JERM Harvester
This file describes how to isolate mRNA from E. coli using the kit from Epicentre, for gene expression analysis via RT-PCR
Creator: Sonja Steinsiek
Submitter: Sonja Steinsiek
This document describes the chemostat conditions to run comparable experiments in different bioreactors in different labs.
Creator: Sonja Steinsiek
Submitter: Sonja Steinsiek
This SOP describes the SUMO procedure for determining B-galactosidase activities.
Creator: Matthew Rolfe
Submitter: Matthew Rolfe