Assays

What is an Assay?

13 Assays visible to you, out of a total of 13

SulfoSys

Kinetic characterisation of glyceraldehyde 3-phosphate dehydrogenase

Submitter: Jacky Snoep

Assay type: Enzymatic Assay

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

SOPs: Cloning and heterologous expression in E. coli, Preparation of cell extracts of recombinant E.coli, Protein purification from E. coli extracts

Data files: GAPDH Kinetic Data

Snapshots: No snapshots

Created: 1st Aug 2013 at 11:37, Last updated: 8th Nov 2017 at 14:21

SulfoSys

Kinetic characterisation of triose phosphate isomerase

Submitter: Jacky Snoep

Assay type: Enzymatic Assay

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

SOPs: Cloning and heterologous expression in E. coli, Preparation of cell extracts of recombinant E.coli, Protein purification from E. coli extracts

Data files: TPI Kinetic Data

Snapshots: No snapshots

Created: 1st Aug 2013 at 14:02, Last updated: 8th Nov 2017 at 14:21

SulfoSys

Kinetic characterisation of fructose 1,6-bisphosphate aldolase phosphatase

Submitter: Jacky Snoep

Assay type: Enzymatic Assay

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

SOPs: Cloning and heterologous expression in E. coli, Preparation of cell extracts of recombinant E.coli, Protein purification from E. coli extracts

Data files: FBPAase Kinetic data

Snapshots: No snapshots

Created: 1st Aug 2013 at 14:05, Last updated: 8th Nov 2017 at 14:21

Temperature Degradation of Gluconeogenic Intermediates

SulfoSys

Temperature degradation of BPG, GAP and DHAP

Submitter: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

SOPs: No SOPs

Data files: Temperature Degradation of Gluconeogenic Interm...

Snapshots: No snapshots

Created: 1st Aug 2013 at 14:15, Last updated: 8th Nov 2017 at 14:21

Experimental Validation Gluconeogenesis in S. solfataricus

SulfoSys

Experimental data for the conversion of 3PG to F6P and the gluconeogenic pathway intermediates

Submitter: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Carbon Loss at High Temperature

Organisms: Sulfolobus solfataricus

SOPs: No SOPs

Data files: Experimental Data for Reconstituted Gluconeogen..., Simulation for Reconstituted Gluconeogenic System

Snapshots: No snapshots

Created: 1st Aug 2013 at 14:22, Last updated: 8th Nov 2017 at 14:21

SulfoSys

Kinetic characterisation of phosphoglycerate kinase

Submitter: Jacky Snoep

Assay type: Enzymatic Assay

Technology type: Initial Rate Experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

SOPs: Cloning and heterologous expression in E. coli, Preparation of cell extracts of recombinant E.coli, Protein purification from E. coli extracts

Data files: PGK Kinetic Data

Snapshots: No snapshots

Created: 1st Aug 2013 at 09:14, Last updated: 8th Nov 2017 at 14:21

SulfoSys

Mathematical model for PGK kinetics, saturation with ADP, ATP, 3PG and BPG.

Submitter: Jacky Snoep

Biological problem addressed: Enzymology

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

Models: PGK Kinetic Model

SOPs: No SOPs

Data files: PGK Kinetic Data, PGK Kinetics Simulation

Snapshots: No snapshots

Created: 1st Aug 2013 at 09:37, Last updated: 8th Nov 2017 at 14:21

Reconstituted Gluconeogenesis System

SulfoSys

In vitro reconstitution of the PGK, GAPHD, TPI and FBPAase enzymes from S. solfataricus

Submitter: Jacky Snoep

Biological problem addressed: Metabolic Network

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

Models: FBPAase Kinetic Model, GAPDH Kinetic Model, Kinetic Model of Temperature Degradation of Glu..., Model of reconstituted gluconeogenesis system i..., PGK Kinetic Model, TPI Kinetic Model

SOPs: No SOPs

Data files: No Data files

Snapshots: No snapshots

Created: 1st Aug 2013 at 12:40, Last updated: 8th Nov 2017 at 14:21

Model Validation Gluconeogenesis in S. solfataricus

SulfoSys

Model prediction of the conversion of 3PG to fructose-6-phosphate and the gluconeogenic pathway intermediates. https://jjj.bio.vu.nl/models/experiments/kouril3_experiment-user/simulate

Submitter: Jacky Snoep

Biological problem addressed: Metabolic Network

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Carbon Loss at High Temperature

Organisms: Sulfolobus solfataricus

Models: Model of reconstituted gluconeogenesis system i...

SOPs: No SOPs

Data files: Experimental Data for Reconstituted Gluconeogen..., Simulation for Reconstituted Gluconeogenic System

Snapshots: No snapshots

Created: 1st Aug 2013 at 12:59, Last updated: 8th Nov 2017 at 14:21

GAPDH Modelling

SulfoSys

Mathematical model for GAPDH kinetics, saturation with BPG, NADPH, NADP, GAP and Pi

Submitter: Jacky Snoep

Biological problem addressed: Enzymology

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

Models: GAPDH Kinetic Model

SOPs: No SOPs

Data files: GAPDH Kinetic Data, GAPDH Kinetics Simulation

Snapshots: No snapshots

Created: 1st Aug 2013 at 14:00, Last updated: 8th Nov 2017 at 14:21

SulfoSys

Mathematical model for TPI kinetics, saturation with GAP and DHAP, and inhibition by 3PG and PEP

Submitter: Jacky Snoep

Biological problem addressed: Enzymology

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

Models: TPI Kinetic Model

SOPs: No SOPs

Data files: TPI Kinetic Data, TPI Kinetics Simulation

Snapshots: No snapshots

Created: 1st Aug 2013 at 14:03, Last updated: 8th Nov 2017 at 14:21

FBPAase Modelling

SulfoSys

Mathematical model for FBPAase kinetics, saturation with DHAP and GAP

Submitter: Jacky Snoep

Biological problem addressed: Enzymology

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

Models: FBPAase Kinetic Model

SOPs: No SOPs

Data files: FBPAase Kinetic data, FBPAase Kinetics Simulation

Snapshots: No snapshots

Created: 1st Aug 2013 at 14:06, Last updated: 8th Nov 2017 at 14:21

Modelling Metabolite Degradation at High Temperature

SulfoSys

Modelling the degradation of BPG, GAP and DHAP at high temperature

Submitter: Jacky Snoep

Biological problem addressed: Metabolism

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model Gluconeogenesis

Organisms: Sulfolobus solfataricus

Models: Kinetic Model of Temperature Degradation of Glu...

SOPs: No SOPs

Data files: Temperature Degradation Kinetics Simulation, Temperature Degradation of Gluconeogenic Interm...

Snapshots: No snapshots

Created: 1st Aug 2013 at 14:18, Last updated: 8th Nov 2017 at 14:21

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