Assays
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Theoretical analysis of hypothetical sigma factor competition. Based on the model 'transcription factor competition' possible dynamics of sigma factor competition are simulated and analysed using Lineweaver-Burk representations.
Submitter: Ulf Liebal
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: The transition from growing to non-growing Baci...
Submitter: Praveen kumar Sappa
Assay type: Glucose Pulse
Technology type: Technology Type
Investigation: The transition from growing to non-growing Baci...
Submitter: Praveen kumar Sappa
Assay type: Transcriptomics
Technology type: Custom Array
Investigation: The transition from growing to non-growing Baci...
Measurement of intra- and extra-cellular metabolome.
Submitter: Ulf Liebal
Assay type: Metabolite Profiling
Technology type: Liquid Chromatography-tandom Mass Spectrometry
Investigation: The transition from growing to non-growing Baci...
Submitter: Sandra Maass
Assay type: Proteomics
Technology type: 2D Gel Electrophoresis
Investigation: The transition from growing to non-growing Baci...
Submitter: Sandra Maass
Assay type: Protein Expression Profiling
Technology type: Mass Spectrometry
Investigation: The transition from growing to non-growing Baci...
Submitter: Sandra Maass
Assay type: Proteomics
Technology type: Mass Spectrometry
Investigation: The transition from growing to non-growing Baci...
We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity. SigmaB itself is placed downstream of Pspac, inducible by IPTG. The lacZ reporter gene is downstream of Pctc promoter. IPTG concentrations of 0.1, 0.2 and 1 mM are added in mid-exponential phase at an OD of appr. 0.3. The whole experiment runs for about eight hours.
Submitter: Ulf Liebal
Assay type: Proteomics
Technology type: Technology Type
Investigation: The transition from growing to non-growing Baci...
