Data files

Model analysis results in binary Matlab format

Mean and SD data for metabolites and biomass, along with metadata used to simulate this experiment.

Complete excel spradsheets for Laurel and Hardy 2, including data for the pgm mutant that were not analysed in the Chew et al. 2017 preprint/publication. Data include fresh and dry biomass, gas exchange, leaf numbers and metabolites in Col0 (WT), prr7prr9, pgm and lsf1 plants. Metabolite data are from plants after 27 days of growth (end of night) and 28 days (end of day and end of night).

RNA levels for control amplicons and multiple clock genes in 2 WT (Col, Ws) and 5 clock mutants of Arabidopsis, in biological duplicates, from three conditions: Diurnal cycle (12L/12D), Extended night (DD), Extended light (LL), harvested every 2 hours. Numbers are in transcript copy per cell, obtained assuming 1 g FW contains 25000000 cells. Comments: Data from LD are concateneted with DD and LL for better visualization. Toc1-101 (col-0) gi-201 (col-0) prr7-3 prr9-1 (col-0) , lhy cca1 (ws) elf3-4 ...

for Chew, Seaton et al. paper Figure 1

For Chew, Seaton et al. Figure 1

Excel spreadsheet with data and simulations used to prepare figures for publication, see Metadata sheet for conditions. Data Fresh (not dry) rosette leaf biomass, measured in samples of 5 plants each on multiple days, as mean and SD; Simulation outputs from FMv2 for Col Wild Type plants, lsf1, and two simulations for prr7prr9 where the mutation affects only starch degradation or both starch degradation and malate/fumarate store mobilisation.

Starch levels in carbon units (not C6) measured on on ...

Experimental data, in MATLAB binary file format.

Model simulation data for Laurel and Hardy 1, in MATLAB binary format.

Biomass and metabolite data for Laurel and Hardy 1.

Batch sample publishing

Batch sample publishing

Batch sample publishing

Analysis of the sequences of PGM1 from several Synechocystis strains revealed a different annotation for Synechocystis sp. PCC 6803, which included a 16-amino acid N-terminal extension that is missing in the other strains. Additionally, the experimentally validated transcriptional start site from Synechocystis sp. PCC 6803 suggests a shorter open reading frame for PGM1, with Met 17 as putative translational start site. To clarify if the N-terminal extension is an annotation error, we prepared ...

To test the effecf of phosphorylation of Ser 47 on PGM1 activity, this residue was exchanged for Ala and Asp and the activity of the recombinant proteins was measured in vitro.

_p_RNAinVAL/_I_01_LabTrials/_S_09_jun2017/_A_03_jun2017-qPCR/

_p_RNAinVAL/_I_01_LabTrials/_S_09_jun2017/_A_02_jun2017-RNAisol/

_p_RNAinVAL/_I_01_LabTrials/_S_07_dec2016/_A_03_dec2016-qPCR/

_p_RNAinVAL/_I_01_LabTrials/_S_07_dec2016/_A_02_dec2016-RNAisol/

_p_RNAinVAL/_I_01_LabTrials/_S_05_jun2016/_A_03_jun2016-qPCR/

_p_RNAinVAL/_I_01_LabTrials/_S_05_jun2016/_A_02_jun2016-RNAisol/

_p_RNAinVAL/_I_01_LabTrials/_S_04_Stages/_A_04_stages-qPCR/

_p_RNAinVAL/_I_01_LabTrials/_S_04_Stages/_A_03_stages-RNAisol/

Batch sample publishing

Batch sample publishing