Shotgun proteomics, toluene

Version 1

Due to its high chemical stability, the biodegradation of benzene is the most challenging amongst hydrocarbons. While oxygen-dependent oxygenases are employed by aerobic bacteria to overcome the energy barrier, the mechanism of anaerobic benzene biodegradation is still enigmatic. The sulfate-reducing, clonal enrichment culture BzS1 is capable of benzene degradation under anoxic conditions. In addition, the alkylbenzene toluene may be used as sole source of carbon and energy. In this project, the ...

Due to its high chemical stability, the biodegradation of benzene is the most challenging amongst hydrocarbons. While oxygen-dependent oxygenases are employed by aerobic bacteria to overcome the energy barrier, the mechanism of anaerobic benzene biodegradation is still enigmatic. The sulfate-reducing, clonal enrichment culture BzS1 is capable of benzene degradation under anoxic conditions. In addition, the alkylbenzene toluene may be used as sole source of carbon and energy. In this project, the ...

The soluble and membrane protein-enriched fraction of clonal enrichment culture BzS1 were prepared and analyzex by means of nanoLC separation of generated peptides coupled to ESI-iontrap MS/MS.

Peaklist files (.mgf) and mascot search files are provided per analysis. Each sample replicate was separated by gel electrophoresis and subdivided into 8 gel-slices. These were subjected to in-gel digest and LC ESI-iontrap MS/MS analyses.