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LC-MS based absolute quantification of intracellular metabolites

SilicoTryp

Intracellular metabolites in T. brucei at different stage of cell growth have been quantified absolutely by isotope ratio based MS technique using uniformly 13C-labelled E. coli extract. This is the case study for method development of absolute quantification for metabolic flux analysis.

Submitter: Dong-Hyun Kim

Assay type: Metabolomics

Technology type: Mass Spectrometry

Investigation: Metabolite profiling, quantification and flux q...

Study: Metabolic flux measurement

Organisms: Trypanosoma brucei : Tbrucei427 (wild-type / wild-type)

SOPs: Sample preparation for intracellular metabolite...

Data files: Absolute intracellular concentrations of metabo...

Snapshots: No snapshots

Created: 11th Dec 2012 at 15:23, Last updated: 8th Nov 2017 at 14:21

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