meta_AType_SxF_lavandulol.txt Version 1
_p_SUSPHIRE/_I_T33_MonoterpenoidsFungi/_S_P5_SxF_lavandulol/
SEEK ID: https://fairdomhub.org/data_files/5998?version=1
Filename:
meta_AType_SxF_lavandulol.txt
Format: Plain text document
Size: 828 Bytes
#Item name Detection of lavandulol in extracts of engineered fungi Lab manager Jaime Primo Sample collection protocol SxF lines provided by Elena Moreno Extraction protocol for liquid cultures Liquid-liquid extraction with 50mL ethyl acetate:hexane (7:3) (x3 times). Concentration up to 1mL and syringe filtration. Extraction protocol for mycelia Solvent extraction with 50 mL ethyl acetate:hexane (7:3) and magnetic agitation for 2 hours. Concentration up to 1mL and syringe filtration. Chromatography protocol 1ul injection, oven temperature program: 60C for 1 min, raise at 10C/min up to 120C, mantained for 4 min; then, raise at 10C/min up to 130C, and finally raised at 20C/min up to 280C, hold for 2 min. Mass spectrometry protocol FS mode:39 to 450 uma at 70 eV. SRM mode: lavandulol - 69-41 at 5eV, 93-77 at 10eV
Export PNG
Creators and Submitter
Views: 655 Downloads: 129
Created: 18th May 2022 at 09:44
Last updated: 7th Nov 2024 at 10:06
AttributionsNone
Version History
Version 1 (earliest) Created 18th May 2022 at 09:44 by Marko Petek
No revision comments
Related items
https://orcid.org/0000-0003-3644-7827PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.
Synthetic biology data management spreadsheet: https://fairdomhub.org/data_files/6036
Programme: NIBSys
Public web page: http://susphire.info/susphireproject/
Start date: 1st Apr 2018
End date: 30th Sep 2021
Short Name: T33_MonoterpenoidsFungi Title: Detection of Coccoidea pheromones or precursors in engineered fungi Description: Detection of lavandulol or lavandulyl acetate in different engineered fungi (SxF) lines by GC-MS-MS Phenodata: ./phenodata_20211223.txt pISA Investigation creation date: 2021-12-23 pISA Investigation creator: Sandra Vacas Principal investigator: Ismael Navarro License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Short Name: P5_SxF_lavandulol Title: Detection of lavandulol in SxF extracts Description: Detection of lavandulol in extracts of engineered fungi by GC-MS-MS Raw Data: pISA Study creation date: 2021-12-23 pISA Study creator: Sandra Vacas Principal investigator: Ismael Navarro License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_T33_MonoterpenoidsFungi
Assays: _A_L_liq-GC-MS, _A_L_myc-GC-MS, _S_P5_SxF_lavandulol-files
Files connected to study: _S_P5_SxF_lavandulol
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_T33_MonoterpenoidsFungi
Study: _S_P5_SxF_lavandulol
