reports/AgroinfGraphs08-12-2019.xlsx

Version 1

National Institute of Biology, Department of Biotechnology and Systems Biology projects

SUSPHIRE aims to provide a sustainable, low-cost biomanufacturing platform for the commercial production of insect pheromones.

Synthetic biology data management spreadsheet: https://fairdomhub.org/data_files/6036

Short Name: T21_SXPsysbio Title: Use a systems biology approach to identify regulatory bottlenecks in SxPv1 Description: Samples from SXPv1.0 plants as well as sister nulls (progeny from the original transgenic event in which the transgene has segregated) and wild type will be grown and leaf samples taken for RNA extraction and profiling of primary metabolites and volatiles (target pheromones as well as potential derivatives) (P1, P5). Phenotypic and GC-MS data will be obtained and analysed from ...

Short Name: P1_SPv1TransientExp Title: Transient expression in Nicotiana benthamiana leaves of constructs for SPv1 Description: The constructs for stable transformation of SPv1.X versions were tested transiently via agroinfiltration of Nicotiana benthamiana leaves. Pheromone content was analysed via GC-MS Raw Data: pISA Study creation date: 2019-12-08 pISA Study creator: ElenaMG Principal investigator: Diego Orzaez License: Creative Commons Attribution 4.0 Sharing permission: Private Upload to ...

Short Name: SPv10EaDActAnalysis-GCMS Assay Class: WET Assay Type: GCMS Title: Transient expression in Nicotiana benthamiana leaves of the moth pheromone pathway with and without EaDAct Description: The effect of EaDAct in pheromone production was studied by agroinfiltrating in Nicotiana benthamiana leaves the first two enzymes (AtrD11 and HarFAR) with or without the last enzyme EaDAct. P19 was also agroinfiltrated as negative control. pISA Assay creation date: 2019-12-08 pISA Assay creator: ElenaMG ...