output/24112017_totRNA_DNase_treated_for_RNAseq_6samples.txt Version 1
_p_RNAinVAL/_I_03_Omics/_S_01_ns-dsRNA_trans/_A_01_RNA-Seq_dsEGFP-NGS/
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output/24112017_totRNA_DNase_treated_for_RNAseq_6samples.txt
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Sample ID User ID Date Time ng/ul A260 A280 260/280 260/230 Constant Cursor Pos. Cursor abs. 340 raw RI0301_water_IND1 after DNase Default 24.11.2017 14:16 90,37 2,259 1,025 2,20 2,37 40,00 230 0,952 0,036 RI0302_water_IND2 after DNase Default 24.11.2017 14:17 92,48 2,312 1,036 2,23 2,47 40,00 230 0,937 0,038 RI0303_water_IND3 after DNase Default 24.11.2017 14:18 76,56 1,914 0,852 2,25 2,37 40,00 230 0,808 0,048 RI0307_dsEGFP_IND1 after DNase Default 24.11.2017 14:19 91,61 2,290 1,025 2,23 2,40 40,00 230 0,954 0,021 RI0308_dsEGFP_IND2 after DNase Default 24.11.2017 14:20 90,35 2,259 1,026 2,20 2,41 40,00 230 0,938 0,016 RI0309_dsEGFP_IND3 after DNase Default 24.11.2017 14:21 88,34 2,209 0,986 2,24 2,38 40,00 230 0,929 0,020
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Created: 12th Nov 2021 at 22:44
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Version 1 (earliest) Created 12th Nov 2021 at 22:44 by Marko Petek
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https://orcid.org/0000-0003-3644-7827PhD in Biotechnology, Research Associate at Department of Biotechnology and Systems Biology, National Institute of Biology
National Institute of Biology, Department of Biotechnology and Systems Biology projects
Projects: HYp - Spatiotemporal analysis of hypersensitive response to Potato virus Y in potato, pISA-tree, MOA - Multiomics analysis of potato response to Potato virus Y (PVY) infection, SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture, INDIE - Biotechnological production of sustainable indole, _p_stRT, ADAPT - Accelerated Development of multiple-stress tolerAnt PoTato, _p_RNAinVAL, tst, tst2, Playground
Web page: http://www.nib.si/eng/index.php/departments/department-of-biotechnology-and-systems-biology
This project’s main objective was to identify novel Colorado potato beetle gene targets for the development of specific RNAi insecticides and further validate prospective targets using transcriptomics. The work included gene selection, establishment of double-stranded RNA (dsRNA) production methodology and the evaluation of dsRNAs’ insecticidal potential in CPB feeding laboratory and field trials.
Programme: NIBSys
Public web page: http://projects.nib.si/rnainval/
Start date: 1st Nov 2014
End date: 31st Oct 2017
Short Name: 03_Omics Title: Omics analysis of RNAi response in CPB Description: Transcriptomics and metagenome changes upon feeding CPB larvae with dsRNA Phenodata: ./phenodata_20210115.txt pISA Investigation creation date: 2021-01-15 pISA Investigation creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Studies: Investigation files, _S_01_ns-dsRNA_trans, _S_02_metagenome_resp
Assays: _A_01-DNAisol, _A_01_RNA-Seq_dsEGFP-NGS, _A_02-DNASeq, _A_02_CLC-RNASeq, _A_03-Centrifuge, _A_04_DE_divers-R, _A_05_extr_reads-rcf, _A_06_extr_bact-assembly, _A_07_allReads_meta-assembly, _I_03_Omics-files, _S_01_ns-dsRNA_trans-files, _S_02_metagenome_resp-files
Short Name: 01_ns-dsRNA_trans Title: CPB transcriptome response to non-specific dsRNA feeding Description: Global transcriptome response of CPB gut tissue to non-specific dsRNA (dsEGFP) feeding Raw Data: pISA Study creation date: 2021-01-15 pISA Study creator: Marko Petek Principal investigator: Marko Petek License: CC BY 4.0 Sharing permission: Private Upload to FAIRDOMHub: Yes
Submitter: Marko Petek
Investigation: _I_03_Omics
Assays: _A_01_RNA-Seq_dsEGFP-NGS, _A_02_CLC-RNASeq, _S_01_ns-dsRNA_trans-files
Short Name: 01_RNA-Seq_dsEGFP-NGS Assay Class: WET Assay Type: NGS Title: Treatment of dsEGFP and water treated samples before sending for RNA-Seq Description: RNA-Seq DNase and cleanup of dsEGFP and water treated CPB larval samples from the june2017 experiment to determine non-specific response of dsRNA consumption pISA Assay creation date: 2021-05-10 pISA Assay creator: Marko Petek Phenodata: ../../phenodata_20210115.txt Featuredata:
Submitter: Marko Petek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: _I_03_Omics
Study: _S_01_ns-dsRNA_trans
