Lipid data file Source: /mnt/users/fabig/DigiSal/crispr_RNAseq/meta/Lipid_data
Description: Lipid data file Source: /mnt/users/fabig/DigiSal/crispr_RNAseq/meta/Lipid_data
SEEK ID: https://fairdomhub.org/data_files/2637
- Knockout omega-3 genes to perturb LC-PUFA metabolism in Salmon
- ELOVL2 Knockout
- FADS Knockout
- CRISPR/Cas9-mediated ablation of elovl2 in Atlantic salmon (Salmo salar L.) inhibits elongation of polyunsaturated fatty acids and induces Srebp-1 and target genes
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Created: 11th Jan 2019 at 14:59
Last updated: 19th Feb 2019 at 11:59

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Version 1 (earliest) Created 11th Jan 2019 at 14:59 by Sahar Hassani
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Institutions: Norwegian University of Life Sciences

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I am the data and model manager for the Digital Salmon
Projects: GenoSysFat, DigiSal, SEEK tutorial for DigiSal
Institutions: Norwegian University of Life Sciences

Salmon farming in the future must navigate conflicting and shifting demands of sustainability, shifting feed prices, disease, and product quality. The industry needs to develop a flexible, integrated basis of knowledge for rapid response to new challenges. The Digital Salmon will be an ensemble of mathematical descriptions of salmon physiology, combining mathematics, high-dimensional data analysis, computer science and measurement technology with genomics and experimental biology into a concerted ...
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Web page: http://tinyurl.com/digisal
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Salmon farming in the future must navigate conflicting and shifting demands of sustainability, shifting feed prices, disease, and product quality. The industry needs to develop a flexible, integrated basis of knowledge for rapid response to new challenges. Project DigiSal will lay the foundations for a Digital Salmon: an ensemble of mathematical descriptions of salmon physiology, combining ...
Programme: The Digital Salmon
Public web page: http://tinyurl.com/digisal
Organisms: Danio rerio, Salmo salar, Oncorhynchus mykiss
The aim of this investigation is to understand molecular mechanisms of PUFA biosynthesis and regulation in order to enable the sustainable use of vegetable oils in aquafeeds as current sources of fish oils are unable to meet increasing demands for omega-3 PUFAs. By generating gene knockouts, we would like to study the genes that are crucial for multi-tissue synthesis of PUFA synthesis in vivo.
Submitter: Sahar Hassani
Studies: ELOVL2 Knockout, FADS Knockout
Assays: Differential expression analysis of genes between FADS-KO and WT, Fatty Acid Analysis, RNAseq, RNAseq-splicing, RNAseq_CountTable, Sanger sequencing
Snapshots: Snapshot 1, Snapshot 2
By generating CRISPR-mediated elovl2 knockout, we are planning to study the crucial role of elovl2 for multi-tissue synthesis of 22:6n-3 in vivo. Endogenously synthesized PUFAs are important for transcriptional regulation of lipogenic genes in Atlantic salmon. This study demonstrates key roles of elovl2 at two penultimate steps of PUFA synthesis in vivo and suggests Srebp-1 as a main regulator of endogenous PUFA synthesis in Atlantic salmon.
Submitter: Sahar Hassani
Investigation: Knockout omega-3 genes to perturb LC-PUFA metab...
Assays: Fatty Acid Analysis, RNAseq, RNAseq-splicing, Sanger sequencing
Snapshots: Snapshot 1
Total lipids are extracted from tissues of white muscle, liver and whole brain from three fish per dietary treatment.
Submitter: Sahar Hassani
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Knockout omega-3 genes to perturb LC-PUFA metab...
Study: ELOVL2 Knockout
Organisms: No organisms
SOPs: No SOPs
Data files: Initial FAD KO_Liver phospholipid fatty acid pr..., Initial FAD KO_White muscle phospholipid fatty ...
Snapshots: No snapshots