Stranded RNAseq libraries were prepared from 1µg total RNA from liver tissue using TruSeq Stranded mRNA library preparation kit (Illumina, San Diego, USA) using double unique indices (#20022371), according to the manufacturer's instruction (Part 15031057 Rev.E). Libraries were sequenced at the Norwegian Sequencing Centre (NSC). All libraries were pooled, and the same pool was sequenced on 4 flow cell lanes on a HiSeq 3000 machine (Illumina), generating 100bp single-end reads. RNA sequencing files (.fastq) were processed in the following manner: (i) All reads from the same individual were merged into one fastq file. (ii) Reads were mapped to the Atlantic salmon genome (GenBank Accession number: GCA_0002333375.4) using STAR (v 2.6.0c) 50. (iii) Read alignments, recorded in BAM format were subsequently used to count uniquely mapped reads per gene using featurecounts (v1.4.4) 51, with the RefSeq gene_ids. Raw illumina reads as well as gene counts are publicly available through ArrayExpress 52 accession E-MTAB-7220.

SEEK ID: https://fairdomhub.org/assays/771

Experimental Assay

Sahar Hassani

Projects: DigiSal

Investigation: Knockout omega-3 genes to perturb LC-PUFA metabolism in Salmon

Study: ELOVL2 Knockout

Assay position:

Assay type: Experimental Assay Type

Technology type: Technology Type

Organisms: No organisms

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Views: 1697

Created: 17th Dec 2018 at 17:12

Last updated: 5th Feb 2019 at 11:46

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