Source data for Figure 3A: Experimental quantitative immunoblotting data.

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Division of labor by dual feedback regulators controls JAK2/STAT5 signaling over broad ligand range

For all experiments, primary CFU-E cells were starved and stimulated with 5 U/ml Epo. At the indicated time points, samples were subjected to quantitative immunoblotting. Experimental data (black circles) with estimated standard errors and trajectories of the best fit (solid lines) are represented. Mass spectrometry data represent replicates of four independent experiments.