SOPs

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Determination of protein phosphorylation levels using Phos-tag™ gels

SUMO

The phosphorylation level of a particular protein can be determined using a procedure based upon western immunoblotting, with Phos-tag™ reagent present in the SDS-PAGE gel. The Phos-tag™ reagent, supplied in the form of Phos-tag™ acrylamide (Wako Pure Chemical Industries, AAL-107), causes proteins to be resolved both on the basis of size and phosphorylation state. This means that phosphorylated and de-phosphorylated forms of the same protein can be distinguished.

Creator: Matthew Rolfe

Submitter: Matthew Rolfe

Investigations: Steady state studies for different oxygen avail...

Studies: The Escherichia coli steady state response to o...

Assays: ArcA phosphorylation at different aerobiosis le...

Created: 18th Jan 2013 at 12:51

SUMO

No description specified

Creator: Katja Bettenbrock

Submitter: The JERM Harvester

Investigations: Analysis of Escherichia coli with linear electr..., Dynamical studies for different oxygen availabi..., Steady state studies for different oxygen avail...

Studies: Analysis of Escherichia coli strains with linea..., Determination of the impact of specific enzyme ..., The Escherichia coli dynamical response to oxyg..., The Escherichia coli steady state response to o...

Assays: Analysis of by-product formation rates in MG1655, Analysis of gene expression rates at different ..., ArcA phosphorylation at different aerobiosis le..., Characterization of E. coli MG1655 and ∆sdhC an..., FNR activity at different aerobiosis levels (st..., Gene expression analysis of mutants with linear..., Physiological measurements from Sheffield chemo..., Transcriptional profiling of E. coli during ana...

Created: 16th Mar 2010 at 17:13

SUMO chemostat conditions

SUMO

This document describes the chemostat conditions to run comparable experiments in different bioreactors in different labs.

Creator: Sonja Steinsiek

Submitter: Sonja Steinsiek

Investigations: Analysis of Escherichia coli with linear electr..., Analysis of the glucose transport in Escherichi..., Dynamical studies for different oxygen availabi..., Steady state studies for different oxygen avail...

Studies: Analysis of Escherichia coli strains with linea..., Characterization of mutant strains with defects..., Determination of the impact of specific enzyme ..., The Escherichia coli dynamical response to oxyg..., The Escherichia coli steady state response to o...

Assays: Analysis of by-product formation rates in MG1655, Analysis of gene expression rates at different ..., ArcA phosphorylation at different aerobiosis le..., Characterization of MG1655 and mutant strains u..., Determination of by-product formation and gluco..., Determination of intracellular metabolite conce..., Determination of intracellular redox state by m..., Gene expression analysis of mutants with linear..., Physiological measurements from Sheffield chemo..., Transcriptional profiling of E. coli during ana...

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Created: 26th Oct 2009 at 14:48

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