Specific activity of Gre2p was measured after incubation with different Tween-20 concentrations (none, 0.01, 0.1, 1.0%). These data are also used to perform a Selwyn Test to determine if enzyme inactivation or slow-onset inhibition occurs. These are the raw data obtained directly from the Synergy UV-Vis Spectrophotometer.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
Investigations: Workflow for characterization of enzymes under ...
Specific activity of Gre2p was measured after incubation with different Tween-20 concentrations (none, 0.01, 0.1, 1.0%). These data are also used to perform a Selwyn Test to determine if enzyme inactivation or slow-onset inhibition occurs. This is a file that can be opened with the Origin Software for analysis and contains data copied in manually from the xlsx.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
Investigations: Workflow for characterization of enzymes under ...
Specific activity of Gre2p was measured after different treatments (none, on ice, at room temperature, in the ITC, stirred, shaken). These data are used to perform a Selwyn Test to determine if enzyme inactivation or slow-onset inhibition occurs. These are the raw data obtained directly from the Synergy UV-Vis Spectrophotometer.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
Investigations: Workflow for characterization of enzymes under ...
Specific activity of Gre2p was measured after different treatments (none, on ice, at room temperature, in the ITC, stirred, shaken). These data are used to perform a Selwyn Test to determine if enzyme inactivation or slow-onset inhibition occurs. This is a file that can be opened with the Origin Software for analysis and contains data copied in manually from the xlsx.
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
Investigations: Workflow for characterization of enzymes under ...
Gre2p activity monitored by NADPH absorbance using different enzyme concentrations. Activity is measured after different storage conditions (treatments) and in presence of different amounts of tween. Also analysis of DLS data of homogeneity of Gre2p samples measured before and after these treatments.
A python workflow is used to analyse the data and create a plot of the data. It requires the following directory structure:
./Script_for_S13andS18.py ./Script_for_S22.py ./Script_for_S15.py
and as ...
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
Investigations: Workflow for characterization of enzymes under ...
Studies: DLS measurements (homogeneity of an enzyme sample), Spectrophotometric Activity Measurements
Assays: DLS measurements in 2 buffers, DLS measurements in KPi Buffer and in KPi buffe..., DLS measurements in KPi buffer with BSA added, Specific activity of Gre2p
SOP describing how to perform specific activity measurements with Gre2p in multiwell plates (96w) using an UV-Vis Plate reader (Synergy H1).
Creators: Gudrun Gygli, Felix Ott
Submitter: Gudrun Gygli
Investigations: Workflow for characterization of enzymes under ...